| 张洪玉,夏磊,彭翔,刘晓勇,赵明军,齐茜.西伯利亚鲟脂肪肝模型的建立.渔业科学进展,2014,35(2):60-65 |
| 西伯利亚鲟脂肪肝模型的建立 |
| Establishment of fatty liver model for sturgeon Acipenser baeri |
| 投稿时间：2013-03-23 修订日期：2014-04-20 |
| DOI：10.11758/yykxjz.20140209 |
| 中文关键词: 鲟鱼 脂肪肝 高脂饲料 |
| 英文关键词: Acipenser baeri Fatty liver High-fat diet |
| 基金项目:中央级公益性科研院所基本科研业务费专项资金项目（2012A0603） |
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| 中文摘要: |
| 选择规格相近\[体重(8.41±0.82)g，体长(10.12±0.44)cm\]的西伯利亚鲟鱼225尾，分为3组，每组3个平行，分别用8%（对照组）、12%（中脂组）、16%（高脂组）油脂含量的饲料投喂。养殖42 d后，测定体长、体重，尾静脉取血，解剖。通过生长指标、生理生化指标及肝脏组织切片观察等评价建模是否成功。结果表明，高脂组生长最快，终末体长、体重显著高于对照组和中脂组（P＜0.05），肝指数显著高于对照组和中脂组（P＜0.05），存活率与另两组差异不显著（P＞0.05）；高脂组血清AST、ALT及TC、TG显著高于对照组（P＜0.05），肝脏TG显著高于中脂组和对照组；对照组肝脏呈暗红色，中脂组肝脏呈浅红色，高脂组呈暗白色，病理切片观察表明,高脂组部分肝细胞坏死，细胞破裂，脂肪颗粒融成一个大的脂肪滴，细胞呈现透明空泡化，细胞核破裂或溶解。通过肝指数、血清生化指标、肝脏TG、肝脏外观颜色及病理组织切片分析，高脂饲料组成功地建立了鲟鱼脂肪肝模型。 |
| 英文摘要: |
| In this study, the fatty liver model for sturgeon Acipenser baeri was successfully established by analyzing hepatosomatic index, serum biochemical indexes, triglyceride in liver, color of liver and pathologic tissue section of sturgeon fed with high-fat diets. Sturgeons at similar body weight (8.41±0.82)g and length (10.12±0.44)cm, were divided into three groups and fed with three diets at oil contents of 8%(control group), 12%(mid-fat), and 16%(high-fat), respectively. After cultured for 42 d, their body length and body weight were measured, and blood was sampled from the caudal vein, then dissection was performed. The growth, serum and liver biochemical indexes and liver biopsy were employed to evaluate the model. Results showed that the final body length, body weight and hepatosomatic index of high-fat treatment was significantly higher than the other treatments (P＜0.05), but the survival rate showed no significant difference (P＞0.05). In high-fat treatment, the serum biochemical indexes, including aspartate aminotransferase, alanine transaminase, triglyceride and total cholesterol were significantly higher than those of control(P＜0.05). Also, the liver triglyceride in high-fat treatment was significantly higher than the other treatments (P＜0.05). The colour of the fish liver was dark-red in control, light red in mid-fat treatment and grey-white in high-fat treatment. Necrosis and ruptured liver cells were found in high-fat treatment, with fat particles merged into bigger fat droplets, cells became transparent and the nucleus were ruptured or dissolved. |
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