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洪泽湖大银鱼(Protosalanx hyalocranius) Cytb和COⅠ基因序列多态性分析
李大命1, 李 康2, 张彤晴1, 唐晟凯1, 刘燕山1, 刘小维1, 穆 欢3, 黄越峰3, 潘建林1
1.江苏省淡水水产研究所 江苏省内陆水域渔业资源重点实验室 南京 210017;2.南京师范大学生命科学学院 南京 210046;3.江苏省洪泽湖渔业管理委员会办公室 淮安 223300
摘要:
为揭示我国洪泽湖大银鱼(Protosalanx hyalocranius)遗传多样性现状,科学保护和合理利用大银鱼种质资源,采用线粒体细胞色素b基因(Cytb)和细胞色素c氧化酶Ⅰ亚基基因(COⅠ)序列,分析了洪泽湖40尾大银鱼的遗传多样性。通过PCR扩增与序列测定分别获得了长度为1141 bp和630 bp的Cytb和COⅠ基因序列。40条Cytb基因序列碱基A、T、G和C的平均含量分别为21.7%、29.3%、16.7%和32.3%,检出6个变异位点,定义7个单倍型,单倍型多样性和核苷酸多样性分别为0.775和0.00129,碱基平均差异数为1.469。40条COⅠ基因序列碱基A、T、G和C的平均含量分别为21.6%、26.0%、19.2%和33.2%,检出5个变异位点,定义6个单倍型,单倍型多样性和核苷酸多样性分别为0.700和0.00207,平均碱基差异数是1.303。Cytb及COⅠ基因单倍型之间的遗传距离较小,且NJ系统进化树聚为一支,说明大银鱼单倍型没有出现遗传分化。Fu’s Fs中性检验结果和碱基歧点分布图均表明,洪泽湖大银鱼近期经历了种群扩张事件。
关键词:  遗传多样性  细胞色素b基因  细胞色素c氧化酶亚基Ⅰ基因  大银鱼  洪泽湖
DOI:10.11758/yykxjz.20160808001
分类号:
基金项目:
Genetic Polymorphism Analysis of Cytb and COⅠGene Sequences of Protosalanx hyalocranius in the Hongze Lake
LI Daming1, LI Kang2, ZHANG Tongqing1, TANG Shengkai1, LIU Yanshan1, LIU Xiaowei1, MU Huan3, HUANG Yuefeng3, PAN Jianlin1
1.Freshwater Fisheries Research Institute of Jiangsu Province, Key Laboratory of Fisheries Resources in Inland Water of Jiangsu Province, Nanjing 210017;2.School of Life Sciences, Nanjing Normal University, Nanjing 210046;3.Hongze Lake Fisheries Administration Committee Office of Jiangsu Province, Huai’an 223300
Abstract:
In order to analyze the genetic diversity of Protosalanx hyalocranius in the Hongze Lake, the mitochondrial cytochrome b (Cytb) and cytochrome c oxidase subunitⅠ(COⅠ) gene sequences were investigated in this study. Using PCR amplification and sequencing, 1141 bp of Cytb and 630 bp of COⅠ sequences were identified. For the Cytb sequence, the contents of A, T, G and C accounted for 21.7%, 29.3%, 16.7%, and 32.3% respectively. Six polymorphic sites and 7 haplotypes were obtained from 40 individuals. The haplotype diversity and nucleotide diversity were 0.775 and 0.00129 respectively. The average nucleotide difference was 1.469. For the COⅠsequence, the contents of A, T, G and C accounted for 21.6%, 26.0%, 19.2% and 33.2% respectively. Five polymorphic sites and 6 haplotypes were obtained from 40 individuals. The haplotype diversity and nucleotide diversity were 0.700 and 0.00207 respectively. The average nucleotide difference was 1.303. The kimura-two-parameters analysis showed that the genetic distances of the haplotypes varied between 0.001 and 0.004 for Cytb, and between 0.002 and 0.005 for COⅠ. Furthermore, the phylogenetic tree constructed with the neighbor-joining (NJ) method indicated that the haplotypes of P. hyalocranius formed one clade, which suggested that there was no genetic differentiation in the haplotypes of Cytb and COⅠof P. hyalocranius. Fu’s Fs values (–0.32836 for Ctyb and –0.52548 for COⅠ) in the neutrality test was negative, but there was no significant difference. The mismatched bases distribution analysis showed a peak type, which indicated that P. hyalocranius in the Hongze Lake had experienced a population expansion.
Key words:  Genetic diversity  Cytb  COⅠ  Protosalanx hyalocranius  Hongze Lake