| 赵 莲,李志辉,张 培,薛 蓓,赖晓芳,张庆起,高 焕,阎斌伦.三疣梭子蟹线粒体基因组SNP在增殖放流家系识别中的应用.渔业科学进展,2018,39(2):156-163 |
| The wild population of Portunus trituberculatus, an important economical species in China, has been dramatically decreased in past decades due to over capturing. To recover wild germplasm resources of P. trituberculatus, the activities of stock enhancement were performed, and the released individuals were about six millions each year in Haizhou Bay of Jiangsu Province. To evaluate the effects of stock enhancement, we utilized molecular markers (mtDNA SNPs) to track the released pedigrees of P. trituberculatus. Previous studies identified 22 SNPs of mtDNA from P. trituberculatus, which were used to trace four released families (on behalf of 0.8~1.2 million released individuals) (A, B, C and D) by HRM (High resolution melt) technology. Nine of 22 SNPs were usable, which resolution melt curve were coincident between female parentages and their offspring. Furthermore, the nine SNPs could be used to distinguish the four families, in which the parentage C, B, A and D could be differentiated by 5, 2, 1 and 1 SNPs, respectively. Our results indicate that the mtDNA SNPs, combined HRM technology, is a wonderful technology to evaluate the effects of stock enhancement of P. trituberculatus. |
