| 许 杨,李 健,崔彦婷,马 骊.脊尾白虾不同蜕皮分期免疫酶、几丁质酶及蜕皮激素的变化.渔业科学进展,2018,39(3):120-125 |
| 脊尾白虾不同蜕皮分期免疫酶、几丁质酶及蜕皮激素的变化 |
| Changes in Immunity-Related Enzymes, Chitinase Activity, and Molting Ecdysteroid Concentration of Exopalaemon carinicauda During Different Molting Stages |
| 投稿时间：2017-02-14 修订日期：2017-03-27 |
| DOI： |
| 中文关键词: 生长蜕皮 生殖蜕皮 免疫酶 几丁质酶 蜕皮激素 |
| 英文关键词: Growth molting Reproductive molting Immune enzyme Chitinase Molting ecdysteroid |
| 基金项目: |
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| 摘要点击次数: 2106 |
| 全文下载次数: 2011 |
| 中文摘要: |
| 为了研究脊尾白虾(Exopalaemon carinicauda)生长蜕皮和生殖蜕皮的差异，本研究对2种蜕皮不同分期(间期C期、前期D期和后期AB期)的非特异性免疫指标[酚氧化酶(PO)、超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、几丁质酶(Chitinase)及蜕皮激素(MH)]进行了差异比较。结果显示，生长蜕皮各分期PO活力差异不显著，生殖蜕皮各分期PO活性呈先降低后升高的趋势，各分期差异显著；间期时，生长蜕皮和生殖蜕皮PO活性差异不显著；前期时，生殖蜕皮显著低于生长蜕皮；后期时，生殖蜕皮显著高于生长蜕皮。生长蜕皮各分期SOD活性逐渐升高，且差异显著，生殖蜕皮后期SOD活性显著低于间期和前期，间期和前期差异不显著；间期时，生殖蜕皮显著高于生长蜕皮，前期二者差异不显著；后期时，生殖蜕皮显著低于生长蜕皮。生长蜕皮各分期AKP、ACP活力均呈先升高后降低的趋势，且各分期差异显著，生殖蜕皮与生长蜕皮变化趋势相同；间期、前期、后期，生长蜕皮都显著高于生殖蜕皮。生长蜕皮时各分期Chitinase活力和MH激素浓度变化趋势相同，呈逐渐升高趋势，且各分期差异显著，生殖蜕皮时，呈先降低后升高趋势，且各分期差异显著；间期和前期，生长蜕皮显著低于生殖蜕皮，后期生长蜕皮显著低于生殖蜕皮。本文首次对脊尾白虾生长蜕皮和生殖蜕皮进行研究，结果表明，生长蜕皮和生殖蜕皮因为卵巢发育而存在明显不同。本研究结果为虾蟹类蜕皮机制的研究、养殖业苗种的育成提供理论基础和科学依据。 |
| 英文摘要: |
| The present study was conducted to evaluate the differences between the growth and reproductive molting of Exopalaemon carinicauda. In this milieu, we analyzed the differences in the representative stages (intermoult C, premoult D, and postmolt AB) of the two types of molting in relation to three parameters: non-specific immune indexes including phenoloxidase (PO), superoxide dismutase (SOD), acid phosphatase (ACP), alkaline phosphatase (AKP), molting-related enzyme (Chitinase), and ecdysone concentration (MH). The activity of PO during different stages of growth molting exhibited no significant difference. While the activity of PO during reproductive molting decreased at first and then increased, and showed significant differences among different stages. At the intermoult stage, the activity of PO of growth and reproductive molting was not significantly different. At the premoult stage, the activity of PO of reproductive molting was significantly lower than that of growth molting. At the postmoult stage, the activity of PO of reproductive molting was significantly higher than that of growth molting. During growth molting, the activity of SOD at all the three stages gradually increased, and demonstrated significant differences. During reproductive molting, the activity of SOD at the postmoult stage was significantly lower than that at the other two stages, and the difference between the intermoult and premoult stages was not significant. At the intermoult stage, the activity of SOD of reproductive molting was significantly higher than that of growth molting. At the premoult stage, there was no significant difference between them, while at the postmoult stage, the SOD activity of reproductive molting was significantly lower than that of growth molting. During growth molting, the activity of AKP and ACP at all the three stages had the same trend, that is they increased at first and then decreased, and the difference was significant. The activity of enzymes during reproductive molting had the same trend as those during growth molting. However, the activity of enzymes at all the three stages during growth molting was significantly higher than that during reproductive molting. At different stages of growth molting, chitinase activity and MH hormone concentration had the same trend, that is they gradually increased, and the difference of the stages was significant. Furthermore, during reproductive molting, they decreased at first and then increased, and the differences between the stages were significant. During the intermoult and premoult stages, chitinase activity and MH hormone concentration of growth molting was significantly lower than those of reproductive molting. During the postmoult stage, chitinase activity and MH hormone concentration of growth molting were significantly lower than those of reproductive molting. The present study conducted is a preliminary study on growth and reproductive molting. The results showed that there was a significant difference between the growth and reproductive molting because of the ovary development. The results provide a theoretical base and a scientific ground for further research on the mechanism of molting and artificial breeding. |
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