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n-3/n-6长链多不饱和脂肪酸对大菱鲆幼鱼脂肪沉积、脂肪吸收及代谢相关酶活性和血清生化指标的影响
谭青1,2, 韩秀杰1,2, 王际英1, 李宝山1, 李学丽1,2, 郝甜甜1, 张利民1
1.山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室 烟台 264006;2.上海海洋大学水产与生命学院 上海 201306
摘要:
为探讨饲料中不同n-3/n-6长链多不饱和脂肪酸(LC-PUFA)对大菱鲆(Scophthalmus maximus)幼鱼生长性能及饲料利用、体组成和消化酶的影响,配制了6种不同n-3/n-6 LC-PUFA比值(29.54,D1组;23.04, D2组;18.97,D3组;9.06,D4组;6.86,D5组;3.87,D6组)的实验饲料。以大菱鲆幼鱼[(12.18±0.01) g]为研究对象, 在循环水养殖系统中开展了为期56 d的养殖实验。结果显示,n-3/n-6 LC-PUFA对大菱鲆幼鱼的成活率(SR)无显著影响(P>0.05);增重率(WGR)随着n-3/n-6 LC-PUFA的降低呈先上升后下降趋势,D6组显著低于其他各组(P<0.05);脂肪沉积率随着饲料中n-3/n-6 LC-PUFA的降低呈下降趋势,且D6组达到最小值,为14.80,显著低于其他各组(P<0.05)。随着饲料中的变化,胰蛋白酶的活性呈先增强后减弱的趋势,且在D4组时达到最大值;脂肪酶活性呈上升趋势。随着饲料中n-3/n-6 LC-PUFA的变化, 脂肪酸合成酶活性呈先上升后下降的趋势,最高值为D4组,显著高于其他各组(P<0.05);葡萄糖-6-磷酸脱氢酶的酶活呈先上升后下降的趋势,D3组为最大值,显著高于其他各组(P<0.05)。随着饲料中n-3/n-6 LC-PUFA的降低,谷草转氨酶呈先上升后下降的趋势;总蛋白、白蛋白和高密度脂蛋白胆固醇均随着饲料n-3/n-6 LC-PUFA的变化呈先上升后下降的趋势,均在D5组时达到最大值。研究表明,饲料中n-3/n-6 LC-PUFA的比例降低会导致大菱鲆幼鱼的脂肪沉积率降低。
关键词:  大菱鲆  n-3/n-6 LC-PUFA  脂肪沉积  消化酶  血清生化指标
DOI:
分类号:
基金项目:
Effects of n-3/n-6 n-3/ n-6 LC-PUFA on fat deposition, digestion, and lipid metabolism in juvenile turbot (Scophthalmus maximus)
TAN Qing1,2, HAN Xiujie1,2, WANG Jiying1, LI Baoshan1, LI Xueli1,2, HAO Tiantian1, ZHANG Limin1
1.Shandong Marine Resource and Environment Research Institute, Shandong Key Laboratory of Marine Ecological Restoration, Yantai 264006;2.2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306
Abstract:
An 8-week feeding trial was conducted in a recirculated system to study the effect of dietary n-3/n-6 long-chain polyunsaturated fatty acid (LC-PUFA) ratios (29.54, D1; 23.04, D2; 18.97, D3; 9.06, D4; 6.86, D5; 3.87, D6) on growth performance, whole-body fatty acid composition, and serum biochemical indices in turbot (Scophthalmus maximus). Six diets were formulated to feed six groups of juvenile turbots [mean initial weight (12.18±0.01) g]. Each diet was randomly fed to triplicate groups of 35 fish per tank. There were no significant differences in the survival rate (SR) of turbot (P>0.05). With decreasing dietary n-3/n-6 LC-PUFA ratios, the weight gain rate (WGR) first increased and then decreased. The WGR of the D6 group was significantly lower than that of the other groups (P<0.05). The lipid deposition rate (LR) reduced with decreasing dietary n-3/n-6 fatty acid ratios, and the D6 group achieved 14.80, which was significant lower than that of the other group (P<0.05). The trypsin content increased as the dietary n-3/n-6 LC-PUFA level decreased from 29.54 to 9.06, and then decreased. Lipase increased with decreasing n-3/n-6 LC-PUFA. As the dietary n-3/n-6LC-PUFA level decreased, fatty acid synthetase and glucose-6-phosphate dehydrogenase first increased and then decreased. When the dietary n-3/n-6 LC- PUFA level was 9.06, fatty acid synthetase levels were significantly higher than those in the other groups (P<0.05). Additionally, glucose-6-phosphate dehydrogenase levels were significantly higher than those in the other groups (P<0.05) when the dietary n-3/n-6 LC-PUFA level was 18.97. Aspartate aminotransferase, total protein, albumin, and high-density lipoprotein cholesterol first increased and then decreased with increasing dietary n-3/n-6 LC-PUFA ratios. These results indicate that, with decreasing dietary n-3/n-6 LC-PUFA ratios, resulting in reduced lipid deposition rate of juvenile turbot.
Key words:  Scophthalmus maximus  n-3/n-6 LC-PUFA  Fat deposition  Digestive enzyme  Serum biochemical indices