| 谭青,韩秀杰,王际英,李宝山,李学丽,郝甜甜,张利民.n-3/n-6长链多不饱和脂肪酸对大菱鲆幼鱼脂肪沉积、脂肪吸收及代谢相关酶活性和血清生化指标的影响.渔业科学进展,2018,39(4):66-73 |
| n-3/n-6长链多不饱和脂肪酸对大菱鲆幼鱼脂肪沉积、脂肪吸收及代谢相关酶活性和血清生化指标的影响 |
| Effects of n-3/n-6 n-3/ n-6 LC-PUFA on fat deposition, digestion, and lipid metabolism in juvenile turbot (Scophthalmus maximus) |
| 投稿时间：2017-04-17 修订日期：2017-05-27 |
| DOI： |
| 中文关键词: 大菱鲆 n-3/n-6 LC-PUFA 脂肪沉积 消化酶 血清生化指标 |
| 英文关键词: Scophthalmus maximus n-3/n-6 LC-PUFA Fat deposition Digestive enzyme Serum biochemical indices |
| 基金项目: |
|
| 摘要点击次数: 2308 |
| 全文下载次数: 1990 |
| 中文摘要: |
| 为探讨饲料中不同n-3/n-6长链多不饱和脂肪酸(LC-PUFA)对大菱鲆(Scophthalmus maximus)幼鱼生长性能及饲料利用、体组成和消化酶的影响，配制了6种不同n-3/n-6 LC-PUFA比值(29.54，D1组；23.04, D2组；18.97，D3组；9.06，D4组；6.86，D5组；3.87，D6组)的实验饲料。以大菱鲆幼鱼[(12.18±0.01) g]为研究对象, 在循环水养殖系统中开展了为期56 d的养殖实验。结果显示，n-3/n-6 LC-PUFA对大菱鲆幼鱼的成活率(SR)无显著影响(P>0.05)；增重率(WGR)随着n-3/n-6 LC-PUFA的降低呈先上升后下降趋势，D6组显著低于其他各组(P<0.05)；脂肪沉积率随着饲料中n-3/n-6 LC-PUFA的降低呈下降趋势，且D6组达到最小值，为14.80，显著低于其他各组(P<0.05)。随着饲料中的变化，胰蛋白酶的活性呈先增强后减弱的趋势，且在D4组时达到最大值；脂肪酶活性呈上升趋势。随着饲料中n-3/n-6 LC-PUFA的变化, 脂肪酸合成酶活性呈先上升后下降的趋势，最高值为D4组，显著高于其他各组(P<0.05)；葡萄糖-6-磷酸脱氢酶的酶活呈先上升后下降的趋势，D3组为最大值，显著高于其他各组(P<0.05)。随着饲料中n-3/n-6 LC-PUFA的降低，谷草转氨酶呈先上升后下降的趋势；总蛋白、白蛋白和高密度脂蛋白胆固醇均随着饲料n-3/n-6 LC-PUFA的变化呈先上升后下降的趋势，均在D5组时达到最大值。研究表明，饲料中n-3/n-6 LC-PUFA的比例降低会导致大菱鲆幼鱼的脂肪沉积率降低。 |
| 英文摘要: |
| An 8-week feeding trial was conducted in a recirculated system to study the effect of dietary n-3/n-6 long-chain polyunsaturated fatty acid (LC-PUFA) ratios (29.54, D1; 23.04, D2; 18.97, D3; 9.06, D4; 6.86, D5; 3.87, D6) on growth performance, whole-body fatty acid composition, and serum biochemical indices in turbot (Scophthalmus maximus). Six diets were formulated to feed six groups of juvenile turbots [mean initial weight (12.18±0.01) g]. Each diet was randomly fed to triplicate groups of 35 fish per tank. There were no significant differences in the survival rate (SR) of turbot (P>0.05). With decreasing dietary n-3/n-6 LC-PUFA ratios, the weight gain rate (WGR) first increased and then decreased. The WGR of the D6 group was significantly lower than that of the other groups (P<0.05). The lipid deposition rate (LR) reduced with decreasing dietary n-3/n-6 fatty acid ratios, and the D6 group achieved 14.80, which was significant lower than that of the other group (P<0.05). The trypsin content increased as the dietary n-3/n-6 LC-PUFA level decreased from 29.54 to 9.06, and then decreased. Lipase increased with decreasing n-3/n-6 LC-PUFA. As the dietary n-3/n-6LC-PUFA level decreased, fatty acid synthetase and glucose-6-phosphate dehydrogenase first increased and then decreased. When the dietary n-3/n-6 LC- PUFA level was 9.06, fatty acid synthetase levels were significantly higher than those in the other groups (P<0.05). Additionally, glucose-6-phosphate dehydrogenase levels were significantly higher than those in the other groups (P<0.05) when the dietary n-3/n-6 LC-PUFA level was 18.97. Aspartate aminotransferase, total protein, albumin, and high-density lipoprotein cholesterol first increased and then decreased with increasing dietary n-3/n-6 LC-PUFA ratios. These results indicate that, with decreasing dietary n-3/n-6 LC-PUFA ratios, resulting in reduced lipid deposition rate of juvenile turbot. |
| 附件 |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
