| 张培,徐莞媛,李志辉,高焕,张庆起,赖晓芳,陈建华,阎斌伦.脊尾白虾不同发育期mtDNA拷贝数变化特征分析.渔业科学进展,2018,39(6):173-179 |
| Mitochondria are important organelles in cells. Mitochondria can produce ATP and provide energy for almost all biological activities. Mitochondria have a relatively independent genetic system, and nuclear genes also encode certain enzymes responsible for mitochondrial DNA (mtDNA) replication and transcription. Therefore, the number and function of mitochondria is essential for embryonic development. Mitochondrial function depends on the integrity of the mitochondrial genome or the regulation of nuclear genome, as well as the mtDNA copy number. Many studies have investigated mtDNA copy numbers in animals, including studies on oocyte maturation, mammalian embryonic development, and human disease, but none have reported the mtDNA copy number in Exopalaemon carinicauda. To study the profiles of mtDNA copy number at different developmental stages of E. carinicauda, and the energy expenditure of tissues at the different stages of development, total DNA from zygote, zoea Ⅰ, zoea Ⅱ, zoea Ⅲ, zoea Ⅳ, zoea Ⅴ, post larvae, and adult shrimps were used to analyze the mtDNA copy number. One TaqMan probe-based real-time PCR was established to assess the mtDNA copy number. Our results showed that the average copy number of mtDNA per cell was 2366, 2648, 2644, 2873, 3559, 9948, 6452, and 8872 in the zygote, zoea Ⅰ, zoea Ⅱ, zoea Ⅲ, zoea Ⅳ, zoea Ⅴ, post larvae, and adults, respectively. These results show that mtDNA copy number is positively correlated with age, and the correlation coefficient (R) was 0.83, i.e. the copy numbers of mtDNA tend to increase with progressing developmental stages of E. carinicauda. |
