| 张乐乐,边力,常青,侯吉伦,陈四清,赵庆,刘琨,葛建龙,刘长琳.圆斑星鲽sox9基因的克隆与表达.渔业科学进展,2018,39(6):72-80 |
| 圆斑星鲽sox9基因的克隆与表达 |
| Cloning and Expression of the sox9 Gene in Spotted |
| 投稿时间：2017-10-10 修订日期：2017-10-30 |
| DOI： |
| 中文关键词: 圆斑星鲽 sox9 性别基因 mRNA表达 |
| 英文关键词: Verasper variegatus sox9 Sex genes mRNA expression |
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| 中文摘要: |
| 本研究筛选到圆斑星鲽(Verasper variegatus)的性别相关基因sox9，并通过RACE技术获得了全长序列，基因全长为3287 bp，包括1431 bp的ORF，编码477个氨基酸，368 bp的5¢ UTR和1488 bp的3¢ UTR。在3¢ UTR中有多聚腺苷酸尾和加尾信号AATAAA。通过荧光定量PCR测定了sox9基因在圆斑星鲽成鱼不同组织中的表达水平，发现sox9基因在圆斑星鲽的脑、眼、鳃、心、肝、胆、肠、精巢、卵巢、肾和肌肉等各个组织中都有不同程度的表达。在鳃、脑和精巢组织中检测到较高水平的sox9转录，其中精巢中的转录水平显著高于其他组织，sox9基因在性腺中的表达显示出性别两相性差异。其在精巢中的表达水平要显著高于卵巢，说明sox9基因与雄性性腺发育相关。通过测定sox9基因在圆斑星鲽幼鱼不同发育时期(20、30、40、50、60、70和80日龄)的表达水平，发现其在20~50日龄表达量逐渐下降，在60日龄时表达量上升，推测表达量上升可能与幼鱼性腺分化相关。 |
| 英文摘要: |
| Significant differences exist in the growth rate and body size of male and female spotted halibut (Verasper variegatus), with the females being significantly larger than the males. All-female cultivation can improve breeding efficiency. Studies investigating the mechanism of sex determination and features of sex differentiation may provide valuable information for all-female cultivation. In this study, we successfully identified the sox9 gene of spotted halibut. The total length is 3287 bp, including a 1431 bp ORF, encoding 477 amino acids; the 5¢ UTR is 368 bp and the 3¢ UTR is 1488 bp. Polyadenylic acid tails and AATAAA caudal signals were identified in the 3¢ UTR. The expression of the sox9 gene differed in the brain, eye, gills, heart, liver, gallbladder, intestine, testis, ovary, kidney, and muscle in spotted halibut. Transcript levels were high in the gill, brain, and testis, and were significantly higher in the testis than in the other tissues. A two-phase gender difference was observed in the gonads; sox9 expression was significantly higher in the testis than in the ovary, indicating that sox9 might be associated with male gonad development. The dynamic expression patterns of the sox9 gene in spotted halibut larvae gradually declined from 20 to 50 d after hatching, with a sudden rise observed after 60 d. This increased expression might be associated with differentiation of larval gonad. |
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