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热应激对银鲫生化指标和HSP70 mRNA表达的影响
周朝伟, 胡续雯, 雷骆, 刘晓庆, 任胜杰, 杨旻珉, 曹高祥, 陈国梁, 邓星星, 李岩, 李路宽
西南大学动物科学学院水产系 重庆 402460
摘要:
本研究旨在探讨热应激对银鲫(Carassius auratus gibelio)生化指标及肝脏HSP70 mRNA表达的影响,选取平均体重为(72.8±10.1) g的银鲫,分别在热应激前(25℃,对照组)、热应激后(32℃,0 h、2 h、6 h、10 h)和恢复适温后6 h (25℃,恢复组)的条件下,测定机体血浆乳酸(LD)、血浆葡萄糖(Glu)、肝糖原(Gly)、己糖激酶(HK)、丙酮酸激酶(PK)和肝脏HSP70 mRNA表达量变化。研究表明,实验组乳酸含量从热应激开始至恢复期,差异始终不显著(P>0.05);实验组血浆葡萄糖水平在热应激后0 h、2 h、6 h,极显著高于对照组(P<0.01);实验组肝糖原水平在热应激0 h时,极显著高于对照组(P<0.01),其余时间点差异不显著(P>0.05);己糖激酶活力在热应激后2 h,显著高于对照组(P>0.05);丙酮酸激酶活力在热应激后6 h,极显著高于对照组(P<0.01);热应激后2 h,肝脏中HSP70 mRNA表达量达到最高(P<0.01),恢复至适温后,表达量与应激前差异(P>0.05)。本研究为进一步探究银鲫的高温应激作用机制及调控机理提供参考数据,同时,也为银鲫养殖和育种等生产实践提供相应参考依据。
关键词:  银鲫  热应激  生化指标  HSP70
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Effects of Heat Stress on Biochemical Indices and HSP70 mRNA Expression in Gibel Carp (Carassius auratus gibelio)
ZHOU Chaowei, HU Xuwen, LEI Luo, LIU Xiaoqing, REN Shengjie, YANG Minmin, CAO Gaoxiang, CHEN Guoliang, DENG Xingxing, LI Yan, LI Lukuan
Department of Fisheries, College of Animal Sciences, Southwest University, Chongqing 402460
Abstract:
The aim of the present study was to assess the effects of heat stress and recovery on biochemical indices and HSP70 mRNA expression in gibel carp (Carassius auratus gibelio). The fishes were selected as experimental animals with an average weight of (72.8±10.1) g under laboratory conditions. Samples used to determine lactic acid (LD) content, glucose (Glu) content, glycogen (Gly) content, hexokinase (HK) activity, pyruvate kinase (PK) activity, and HSP70 mRNA expression were obtained before heat stress (25℃, the control group), 0, 2, 6, and 10 h after stress (32℃), and heat stress recovery (25℃, HSR), respectively. The results revealed no significant difference in LD content compared with that in the control group (P>0.05). Glu content was significantly increased at 0, 2, and 6 h (P<0.01). Gly was significantly increased at 0 h (P<0.01). HK activity was significantly increased at 2 h (P<0.01). PK activity significantly increased at 6 h (P<0.01). Meanwhile, the highest HSP70 mRNA expression was observed at 2 h under heat stress (P<0.01). Following HSR (25℃, 6 h), the HSP70 mRNA expression level recovered to the pre-stress levels (P>0.05). The results provide basic data for research on the mechanism of high temperature adaption in gibel carp, as well as a scientific basis for acute temperature change in aquaculture and breeding.
Key words:  Carassius auratus gibelio  Heat stress  Biochemical indices  HSP70