| 董丽君,罗 坤,曹家旺,陈宝龙,栾 生,曹宝祥,隋 娟,孟宪红.凡纳滨对虾商业苗种抗WSSV性能比较.渔业科学进展,2019,40(1):69-75 |
| The white spot syndrome virus (WSSV) is a highly virulent pathogen in crustaceans. The purpose of this study was to compare the WSSV-resistant traits of Pacific white shrimp Litopenaeus vannmei from different commercial brands. After detecting the infection status of eight different pathogens, including WSSV, infectious hypodermal and hematopoietic necrosis virus (IHHNV), taura syndrome virus (TSV), acute hepatopancreatic necrosis disease causing-Vibrio parahaemolyticus (VPAHPND), Yellow head virus (YHV), infectious myonecrosis virus(IMNV), Enterocytozoon hepatopenaei (EHP), and covert mortality nodavirus (CMNV), a quantitative feeding method to conduct a WSSV infection experiment on six commercial brands of juvenile L. vannamei (named Hainan Z, Hainan S, Guangzhou P, Guangzhou Z, Huanghua R, and Dongying M in the present study). The WSSV-resistant traits of each commercial brand were evaluated using the average survival time, survival rate, and cumulative mortality after infection. The results showed that no WSSV was found in shrimp of the six commercial brands, whereas some individuals were positive for EHP and CMNV. The average survival time for Hainan Z, Hainan S, Guangzhou P, Guangzhou Z, Huanghua R, and Dongying M was (150.18±25.92) h, (99.51±28.04) h, (122.32±43.59) h, (93.50±34.77) h, (100.99±26.84) h, and (71.70±23.79) h, respectively. The average survival time of Hainan Z was significantly higher than that of the other five brands. The average survival time of Guangzhou P was lower than that of Hainan Z, and was significantly higher than that of Dongying M, Guangzhou Z, Hainan S, and Huang Hua R. Dongying M had a significantly lower survival time than that of the other five brands. There was no significant difference between Guangzhou Z, Hainan S, and Huanghua R (P>0.05). The mortality peak for Hainan Z started on the 6th day and continued to the 7th day; whereas that of Dongying M was on the 4th day. The mortality peak for Hainan Z was 2 to 3 d later than that of Dongying M. At the end of the infection experiment, the survival rate of Hainan Z and Guangzhou P was the highest and was 72.5%. The survival rates for Dongying M and Huanghua R were the lowest, at 39.6% and 38.7%, respectively. The results suggested that Hainan Z and Guangzhou P have the strongest resistance against WSSV. The results could provide basic data for the selective breeding of L. vannamei. |
