文章摘要
王成强,李宝山,王际英,黄炳山,孙永智,郝甜甜,马长兴,周莹.饲料中添加枯草芽孢杆菌和酵母培养物对珍珠龙胆石斑鱼幼鱼生长、血清生化指标及抗氧化能力的影响.渔业科学进展,2019,40(4):47-56
饲料中添加枯草芽孢杆菌和酵母培养物对珍珠龙胆石斑鱼幼鱼生长、血清生化指标及抗氧化能力的影响
Effects of Dietary Bacillus subtilis and Yeast Culture on Growth, Serum Biochemical Indices and Antioxidant Capacity of Juvenile Hybrid Grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂)
投稿时间:2018-05-29  修订日期:2018-06-21
DOI:
中文关键词: 珍珠龙胆石斑鱼  枯草芽孢杆菌  酵母培养物  生长  抗氧化能力  抗病力
英文关键词: Epinephelus fuscoguttatus♀×E. lanceolatus♂  Bacillus subtilis  Yeast culture  Growth  Antioxidant capacity  Disease resistance
基金项目:烟台市科技计划项目(2016ZH068)、山东省重点研发计划(2016GSF115005)和山东省自然科学基金(ZR2015CQ023)共同资助
作者单位
王成强 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室 烟台 264006 
李宝山 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室 烟台 264006 
王际英 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室 烟台 264006 
黄炳山 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室 烟台 264006 
孙永智 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室 烟台 264006 
郝甜甜 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室 烟台 264006 
马长兴 上海海洋大学水产与生命学院 水产科学国家级实验教学示范中心 上海 201306 
周莹 上海海洋大学水产与生命学院 水产科学国家级实验教学示范中心 上海 201306 
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中文摘要:
      为探究饲料中添加枯草芽孢杆菌(Bacillus subtilis)和酵母培养物对珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×E. lanceolatus♂)幼鱼生长、血清生化指标、抗氧化能力和抗病力的影响,采用2×3双因子实验设计,在基础饲料中添加0 (B0)、0.5% (B1)和1.0% (B2)的枯草芽孢杆菌制剂,同时在每个枯草芽孢杆菌水平添加0 (Y0)、0.5% (Y1)和1.0% (Y2)的酵母培养物,制作9组等氮、等脂的实验饲料,饲喂初始体重为(23.41±0.47) g珍珠龙胆石斑鱼幼鱼56 d。结果显示,1)枯草芽孢杆菌和酵母培养物的交互作用对幼鱼存活率(SR)和增重率(WGR)均无显著影响(P>0.05),幼鱼WGR在Y1B1和Y2B2组处于较高水平,显著高于对照组和Y2B2组(P<0.05)。2)枯草芽孢杆菌和酵母培养物的交互作用对血清谷草转氨酶(AST)和碱性磷酸酶(AKP)影响显著(P<0.05),血清ALT和AKP活力在Y1B1和Y1B2组处于较低水平,且显著低于对照组和Y2B2组(P<0.05)。3)枯草芽孢杆菌和酵母培养物的交互作用对肠道超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量影响显著(P<0.05)。肠道SOD、过氧化氢酶(CAT)活力和总抗氧化能力(T-AOC)在Y1B1和Y1B2组处于较高水平,显著高于对照组和Y2B2组(P<0.05),而MDA含量同SOD活力呈相反的变化趋势(P<0.05)。4)枯草芽孢杆菌和酵母培养物的交互作用对鳗弧菌(Vibrio anguillarum)攻毒后幼鱼累积存活率无显著影响(P>0.05)。鳗弧菌攻毒后幼鱼累积存活率在Y1B1组最高,显著高于对照组(P<0.05)。
英文摘要:
      A 2×3 two-factorial experiment was designed to investigate the effects of dietary administration of Bacillus subtilis and yeast culture on growth, serum biochemical indices, antioxidant capacity, and disease resistance of juvenile hybrid grouper (Epinephelus fuscoguttatus♀×E. lanceolatus♂) [initial weight: (23.41±0.47) g]. Nine isonitrogenous and isoenergetic diets were formulated with three levels of B. subtilis [0 (control), 0.5%, and 1.0%], and each B. subtilis level was formulated with three yeast culture levels [0 (control), 0.5%, and 1.0%]. Juvenile hybrid groupers were fed for 8 weeks. The results showed that the interaction between B. subtilis and yeast culture had no significant effects on the survival (SR) and weight gain rate (WGR) of grouper (P>0.05). The WGR of Y1B1 and Y1B2 groups was significantly higher than that of the control and Y2B2 groups (P<0.05).The interaction between B. subtilis and yeast culture had a significant effect on the aspartate aminotransferase (AST) and alkaline phosphatase (AKP) in the serum of grouper (P<0.05); the AST and AKP activities in the serum of Y1B1 and Y1B2 groups were significantly lower than those in the control and Y2B2 groups (P<0.05). The interaction between B. subtilis and yeast culture had a significant effect on the superoxide dismutase (SOD) and malondialdehyde (MDA) levels in the intestinal tract of grouper (P<0.05), but no significant interaction with catalase (CAT) or total antioxidant capacity (T-AOC) in the intestinal tract (P>0.05). The activities of SOD, CAT, and T-AOC in the intestinal tract of the Y1B1 and Y1B2 groups were significantly higher than those in the control and Y2B2 groups (P<0.05), but the MDA content in the intestinal tract showed an opposite trend to SOD (P<0.05). There was no interaction between the effects of dietary B. subtilis and yeast culture on the cumulative survival rate of juveniles challenged with Vibrio anguillarum (P>0.05). The highest value of cumulative survival rate of juveniles was found in the Y1B1 group, which was significantly higher than that in the control group (P<0.05). In conclusion, supplementation of 0.5% or 1.0% B. subtilis and 0.5% yeast culture in diets could promote WGR, antioxidant capacity, and disease resistance in juvenile hybrid grouper.
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