文章摘要
魏平平,李鑫,张俊鹏,费凡,马贺,高东奎,宋昌斌,陈涛,刘鹰.LED光谱对红鳍东方鲀仔稚鱼形态性状及生长相关基因表达的影响.渔业科学进展,2020,41(1):162-168
LED光谱对红鳍东方鲀仔稚鱼形态性状及生长相关基因表达的影响
Effects of LED spectra on morphological character and gene expression of growth in Takifugu rubripes larvae
投稿时间:2018-12-07  修订日期:2019-01-02
DOI:
中文关键词: LED光谱  红鳍东方鲀仔稚鱼  形态性状  生长相关基因的表达
英文关键词: LED Spectrum  Takifugu rubripes larvae  Morphological character  Gene expression of growth
基金项目:
作者单位
魏平平 大连海洋大学 大连 116023
辽宁省水产设施养殖与装备工程研究中心 大连 116023 
李鑫 大连海洋大学 大连 116023
辽宁省水产设施养殖与装备工程研究中心 大连 116023 
张俊鹏 大连海洋大学 大连 116023
辽宁省水产设施养殖与装备工程研究中心 大连 116023 
费凡 大连海洋大学 大连 116023
辽宁省水产设施养殖与装备工程研究中心 大连 116023 
马贺 大连海洋大学 大连 116023
辽宁省水产设施养殖与装备工程研究中心 大连 116023 
高东奎 大连海洋大学 大连 116023
辽宁省水产设施养殖与装备工程研究中心 大连 116023 
宋昌斌 中国科学院半导体研究所 北京 100083 
陈涛 深圳市超频三科技股份有限公司 深圳 518000 
刘鹰 大连海洋大学 大连 116023
青岛海洋科学与技术试点国家实验室 青岛 266000
辽宁省水产设施养殖与装备工程研究中心 大连 116023 
摘要点击次数: 1297
全文下载次数: 1966
中文摘要:
      设置4种LED光谱环境,分别为蓝(λ450 nm)、绿(λ525 nm)、黄(λ590 nm)和白(λ400~780n m),水温控制在(22±1)℃,光周期为16L:8D,光强设为200 mW/m2。研究光谱对红鳍东方鲀(Takifugu rubripes)受精卵孵化和孵化后1~18 d (即实验第6~23天)仔稚鱼形态性状(全长、体长、躯干长、尾长、头长、眼径和体高)以及生长相关基因,包括生长激素(GH)、生长激素受体1型(GHR1)、类胰岛素生长因子1型(IGF1)基因相对表达量的影响。结果显示,蓝、绿、黄光处理组的受精卵比白光处理组早2 d孵化;在实验结束时,蓝光处理组中,仔稚鱼的全长、体长、躯干长和尾长生长最快,黄光处理组中,头长、眼径和体高生长最快。对于生长相关基因,蓝光下的GH基因表达量显著高于黄、白光组(P<0.05),但与绿光组无显著性差异;不同光谱下,GHR1、IGF1基因的表达量无显著性差异。结果表明,蓝光、绿光和黄光促进红鳍东方鲀受精卵的孵化,蓝光有利于红鳍东方鲀仔稚鱼的生长发育。本文研究了不同光谱对红鳍东方鲀仔稚鱼早期发育的形态性状及生长相关基因表达的影响,为养殖厂对红鳍东方鲀育苗提供科学的参考依据。
英文摘要:
      Four LED spectral parameters were set, which were blue (λ450 nm), green (λ525 nm), yellow (λ590 nm), and white (λ400~780 nm), water temperature was controlled at (22±1)℃, photoperiod was 16L:8D. The light intensity was set at 200 mW/m2. The results showed that the fertilized eggs of Takifugu rubripes under the blue, green and yellow light hatched 2 days earlier than the white light. At the end of the experiment, the full length, body length, trunk length and tail length of the larvae under the blue light were the most. Under yellow light, the head length, eye diameter, and body height showed the fastest growth. For growth genes, the expression of growth hormone (GH) gene under the blue light was significantly higher than yellow and white light group (P<0.05), but there was no significant difference with green light group. There was no significant difference in gene expression for growth hormone receptor 1 (GHR1) and insulin-like growth factor type (IGF-1) under different spectra, but the expression of GH gene under blue light was the highest. The results showed that LED spectrum (blue, green, and yellow) promoted the hatching of the fertilized eggs of T. rubripes, and the blue spectrum is beneficial to the growth and development of the T. rubripes larvae. The paper provided a scientific reference for the incubation of T. rubripes.
附件
查看全文   查看/发表评论  下载PDF阅读器
关闭