文章摘要
范婷婷,冯艳微,刘文芬,王卫军,杨建敏.基于微卫星亲权鉴定技术的短蛸性选择研究.渔业科学进展,2020,41(2):168-175
基于微卫星亲权鉴定技术的短蛸性选择研究
Sexual Selection in Octopus ocellatus Based on Microsatellite Paternity Identification Technology
投稿时间:2019-01-18  修订日期:2019-02-21
DOI:
中文关键词: 短蛸  微卫星标记  亲权鉴定  交配模式  性选择
英文关键词: Octopus ocellatus  Microsatellite markers  Paternity testing  Mating pattern  Sexual selection
基金项目:
作者单位
范婷婷 上海海洋大学 水产科学国家级实验教学示范中心 上海 201306
山东省海洋资源与环境研究院 山东省海洋生态资源重点修复实验室 烟台 264006 
冯艳微 山东省海洋资源与环境研究院 山东省海洋生态资源重点修复实验室 烟台 264006 
刘文芬 中国水产科学研究院长江水产研究所 农业农村部水产品质量安全风险评估实验室 武汉 430070 
王卫军 鲁东大学农学院 烟台 264025 
杨建敏 鲁东大学农学院 烟台 264025 
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中文摘要:
      为研究短蛸(Octopus ocellatus)的性选择行为,本研究利用14个多态性微卫星标记,对3个母本、176个子代及17个候选父本进行亲权鉴定,对3个家系中有子代和无子代雄蛸、子代比例高和子代比例低的雄蛸的各形态参数进行t检验,并对子代比例高和子代比例低的父本与母本的遗传相似性与遗传距离进行分析。结果显示,短蛸为多雌多雄的交配模式,为真正意义的雌性混交动物;雄蛸有无子代与其形态参数无相关性;子代比例与父本的形态大小参数无关,而与父母本的遗传相似性和遗传距离有关,父母本遗传相似性越高,遗传距离越小,后代比例越高。本研究为揭示短蛸交配前后的性选择机制提供了重要线索,也为海洋头足类动物的性选择机制研究提供了基础资料。
英文摘要:
      Octopus ocellatus, affiliated to Octopodidae under the phylum Mollusca, is one of the most important economic species along the northern coastal areas of China. It has been considered as a candidate for aquaculture enhancement and release owing to its short life span and rapid growth. However, little is known about sexual selection before and after copulation in this species. To explore the mechanism of sexual selection in O. ocellatus, 11 morphological parameters of 36 adult octopuses were first measured and analyzed. Afterwards, 14 polymorphic microsatellite DNA markers were used to identify the paternity of 176 offspring and 3 female and 17 male parent candidates. The morphological parameters of male parents with and without offspring and those with high and low proportion of offspring were analyzed in three families by the t-test. The genetic similarity between female and male parents which had high and low percentage of offspring was also calculated. The results showed that there was a strong correlation between mantle length, wrist 2, wrist 6, and body weight in male octopuses, and that sexual dimorphism in the total weight and mantle width between male and female octopuses existed. Paternity testing revealed all three females mated with more than two males and 10 males mated with at least two females, which confirmed the polygyny and polyandry pattern in this species. There was no correlation between male octopuses with or without offspring and their morphological parameters. Moreover, the proportion of offspring was not related to the morphological parameters of males but was correlated to the genetic similarity and genetic distance between female and male parents—higher the genetic similarity, higher the proportion of offspring. This study provides a scientific basis for the resource protection and artificial breeding of O. ocellatus, and also, important information for research on the mechanism of sexual selection in marine cephalopods.
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