| 魏智薪,辛鲁生,白昌明,李亚楠,张淑敏,李成华,王崇明.魁蚶Ets家族9个基因的克隆及其在病毒感染应答中的表达分析.渔业科学进展,2019,40(6):121-130 |
| Ets transcription-factor networks represent a model for how combinatorial gene expression is achieved. The characteristic feature of Ets factors is the conserved ETS domain (Helix-Turn-Helix). Thus, the Ets proteins bind to a core GGAA/T consensus sequence and regulate expression of several genes and play an important role in various cellular functions (mitosis, growth, development, differentiation, and apoptosis) and the regulation of immunity. In this experiment, 9 Ets genes (named ETS 1~ETS9, respectively) of the Ark clam (Scapharca broughtonii) were successfully obtained by gene cloning technology, and the open reading frames (ORFs) were 1065 bp, 1290 bp, 1569 bp, 912 bp, 1344 bp, 1404 bp, 1521 bp, 1968 bp, and 1191 bp, respectively. Moreover, they encoded respectively 354, 429, 522, 303, 447, 468, 506, 655, and 396 amino acids. Evolutionary relationships of taxa showed that all genes in this chapter belonged to the Ets family genes. The qPCR detection showed that the expression of two Ets genes (ETS-1, ETS-3) was significantly increased. Thus, the present study showed that the Ark clam ETS-1 and ETS-3 are involved in the replication process of the OsHV-1 under high temperature conditions (16±2)℃. In conclusion, the results of this study provide a scientific basis for further study concerning the large number of deaths of Ark clams due to infection with OsHV-1 in summer. |
