| 项子龙,刘长琳,张金勇,胡鹏,何暮春,柳淑芳,庄志猛,薛同明.金乌贼端粒酶逆转录酶基因鉴定和组织差异表达分析.渔业科学进展,2020,41(3):158-164 |
| 金乌贼端粒酶逆转录酶基因鉴定和组织差异表达分析 |
| Gene Identification and Tissue Differential Expression Analysis of Sepia esculenta Telomerase Reverse Transcriptase |
| 投稿时间：2019-03-26 修订日期：2019-04-21 |
| DOI：10.19663/j.issn2095-9869.20190326001 |
| 中文关键词: 金乌贼 TERT基因 qRT-PCR 系统进化树 基因差异表达分析 |
| 英文关键词: Sepia esculenta TERT gene qRT-PCR Phylogenetic tree Gene differential expression analysis |
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| 中文摘要: |
| 本研究从金乌贼(Sepia esculenta)转录组中筛选出疑似TERT (Telomerase reverse transcriptase)基因序列，通过系统进化和同源序列分析，鉴定出该基因为金乌贼TERT基因，序列长度为2415 bp。采用实时荧光定量PCR技术，检测和分析金乌贼TERT基因在不同组织、不同发育时期和不同性别的相对表达量差异。结果显示，与产卵期相比，濒死期的心、肝、胰、鳃、性腺、食道上神经团(除去视腺和视叶后的脑组织)等6个组织的TERT基因表达量呈显著下调趋势，推测与濒死期的器官组织分裂和增殖能力下降有关。TERT基因在金乌贼不同组织中具有明显的组织特异性，说明金乌贼各组织器官的衰老状态并不一致。本研究结果可为深入研究头足类生殖与衰老等生命过程的端粒酶调控机制提供基础数据。 |
| 英文摘要: |
| Telomerase reverse transcriptase (TERT) gene is a key structure and major regulatory subunit of telomerase. TERT synthesizes telomeric DNA repeats by reverse transcription of telomerase RNA template sequences and adds them to the ends of chromosomes to maintain and extend the ends. Golden cuttlefish (Sepia esculenta) is an annual species that dies quickly after spawning. This characteristic makes it a potential research object for the study of reproduction and aging. In this study, the phylogenetic and homologous sequence analysis revealed that the gene was a Sepia esculenta TERT gene with a sequence length of 2415 bp. Quantitative Real-time PCR (qRT-PCR) was used to detect the relative expression of TERT gene in the growth stages, spawning stages and aging stages of S. esculenta, and to analyze life cycle differences and gender differences. The research results show that TERT gene expression in six tissues, including heart, liver, pancreas, gill, gonad and esophageal nerve mass, showed a downward trend, which was supposed to be related to the decline of organ division and proliferation in the near-death stage. TERT gene has obvious specificity in different tissues of, Sepia esculenta. It shows that the aging status of different tissues and organs of Sepia esculenta is different. The results of this study can provide basic data for the further study of the regulation mechanism of Telomerase in reproduction and senescence of cephalopod. |
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