文章摘要
姜燕,徐永江,于超勇,柳学周,王滨,郑炜强,官曙光,史宝,陈佳,柯巧珍.大黄鱼消化道菌群结构、消化酶和非特异性免疫酶活力分析.渔业科学进展,2020,41(5):61-72
大黄鱼消化道菌群结构、消化酶和非特异性免疫酶活力分析
Analysis of Microbiota Structure, Digestive Enzyme and Nonspecific Immune Enzyme Activity in the Gastrointestinal Tract of Large Yellow Croaker
投稿时间:2019-05-15  修订日期:2019-07-11
DOI:
中文关键词: 大黄鱼  消化道  菌群  消化酶  非特异性免疫酶  养殖模式
英文关键词: Large yellow croaker  Gastrointestinal tract  Microbiota  Digestive enzymes  Nonspecific immune enzymes  Culture mode
基金项目:
作者单位
姜燕 大黄鱼育种国家重点实验室 宁德市富发水产有限公司 宁德 352103中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071 
徐永江 中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071 
于超勇 山东省海洋生物研究院 青岛 266104 
柳学周 大黄鱼育种国家重点实验室 宁德市富发水产有限公司 宁德 352103中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071 
王滨 中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071 
郑炜强 大黄鱼育种国家重点实验室 宁德市富发水产有限公司 宁德 352103 
官曙光 山东省海洋生物研究院 青岛 266104 
史宝 中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071 
陈佳 大黄鱼育种国家重点实验室 宁德市富发水产有限公司 宁德 352103 
柯巧珍 大黄鱼育种国家重点实验室 宁德市富发水产有限公司 宁德 352103 
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中文摘要:
      为研究大黄鱼(Larimichthys crocea)消化道的菌群结构、消化酶和非特异性免疫酶活力特征,本研究采用高通量测序技术系统分析大黄鱼胃、幽门盲囊和肠道中菌群组成及分布,并对比研究工厂化养殖和网箱养殖模式下的消化道菌群;同时,结合生化分析方法解析2种模式下消化道消化酶和非特异性免疫酶活力特征。结果显示,2种养殖模式下,菌群多样性随消化道延伸呈下降趋势;乳杆菌科(Lactobacillaceae(f))、Fructobacillus、黄杆菌属(Flavobacterium)等代表的菌属为共有优势菌群。其中,拟杆菌属(Bacteroides)和Anaerostipes等的丰度随消化道延伸呈下降趋势,而乳杆菌科、E01_9C_26_marine_group(o)所代表的菌属及黄杆菌属等则相反;普氏菌属(Prevotella_9)、乳杆菌科代表的菌属为2种模式养殖大黄鱼的主要差异菌属。工厂化养殖条件下,幽门盲囊和肠道中的菌群组成及其参与营养和免疫相关代谢通路的基因数目差异不显著(P>0.05),但与胃部的菌群组成和相关代谢通路基因数目存在明显差异;而网箱养殖大黄鱼胃部与幽门盲囊和肠道的菌群结构及相关代谢通路基因数目差异相对较小。2种养殖模式下的大黄鱼消化道菌群与饲料菌群相近。另外,胃和幽门盲囊也具有非特异性免疫酶活性,说明,整个消化道还具有一定的化学免疫屏障作用。本研究结果将为大黄鱼健康养殖提供基础参考,并为消化道菌群生理功能探讨提供理论依据。
英文摘要:
      The aim of this project was to investigate the characteristics of the microbiota structure and the activity of digestive and nonspecific immune enzymes in the gastrointestinal tract of large yellow croaker (Larimichthys crocea). We systematically analyzed the composition and distribution of microbiota in the stomach, pyloric caecum, and gut of large yellow croaker using high-throughput sequencing. Moreover, we conducted comparative analysis of the gastrointestinal tract microbiota in large yellow croakers cultured via the industrialized or net cage culture pattern. Under these two aquaculture patterns, we analyzed the activity of digestive and nonspecific immune enzymes in the gastrointestinal tract through biochemical methods. The microbiota diversity in the gastrointestinal tract declined under both aquaculture patterns. The genera including Lactobacillaceae(f), Fructobacillus and Flavobacterium, and etc, were the shared and dominant microbiota. The abundances of Bacteroides and Anaerostipes declined from the beginning of the gastrointestinal tract to the end, while those of Flavobacterium and genera represented by Lactobacillaceae (f) and the E01_9C_26_marine_group increased under both aquaculture patterns. Genera belonging to Prevotella_9 and Lactobacillaceae(f) were the main different species between these two aquaculture patterns. Under the industrialized pattern, the composition of microbiota and number of genes involved in metabolic pathways associated with nutrition and immunity were not significantly different between the pyloric caecum and gut (P>0.05), while these values obviously different from those in the stomach. In contrast, under net cage culture, differences between the stomach and pyloric caecum or gut decreased. The microbiota composition of the gastrointestinal tract of large yellow croakers cultured with these two patterns was similar to that of the feed. Additionally, the stomach and pyloric caecum exhibited nonspecific immune enzyme activities, indicating that the entire gastrointestinal tract functions as a chemical immune barrier. These results can serve as a basis for healthy large yellow croaker cultures and provide a theoretical foundation for studying the physiological functions of the gastrointestinal tract microbiota.
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