| 陈梓聪,陈丕茂,袁华荣,冯雪,佟飞,陈文静,龙鑫玲,张皓铭.恢复时间对力竭训练后的斑节对虾幼虾的抗氧化功能的影响.渔业科学进展,2020,41(6):58-65 |
| Exhaustive exercise often occurs in shrimp farming and in the wild. Giant tiger prawn (Penaeus monodon) is an important species in aquaculture and proliferation in China's coastal areas. The study used dip nets to chase juvenile P. monodon in the indoor sink, simulated the exhaustive exercise experiment, and explored the recovery process of antioxidant capacity after exhaustive exercise; however, there was no report on the physiological recovery process after the exhaustive exercise of P. monodon. The results showed the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) content in juvenile P. monodon at 0 h after exhaustive exercise. No significant difference from the control group was observed. At 1~12 h, the activities of SOD, CAT, POD, MDA content, and T-AOC continued to decrease, whereas the activity of GSH-Px did not change significantly. At 24 h, the activities of SOD, CAT, and POD were significantly lower than those of the control group, and the activity of GSH-Px, MDA content, and T-AOC were significantly higher than those of the control group. At 72 h, the activities of SOD and CAT were still significantly lower than those in the control group. The activities of GSH-Px, POD, and MDA content were restored in the control group, and T-AOC activity was still significantly higher compared to that in the control group. This indicated that the enzymatic antioxidant system of P. monodon was not activated during exhaustive exercise. At 24 h after exhaustive exercise, the antioxidant capacity at 72 h was still at a high level. It was observed that the enzymatic antioxidant system was not activated in juvenile P. monodon during exhaustive exercise. The antioxidant capacity of juvenile P. monodon is lower within 24 h after exhaustive exercise, then increases significantly after 24 h of exhaustion and remained at that level for a long time. Within 24 h after exercise, further stimulation of juvenile P. monodon should be avoided. |
