| 刘峻宇,刘均辉,孔杰,代平,于洋,孟宪红,罗坤,曹宝祥,陈宝龙,高焕,栾生.凡纳滨对虾SNP标记开发与家系亲缘关系验证分析.渔业科学进展,2021,42(1):108-116 |
| The Large-scale family-based selective breeding can effectively increase genetic gain in shrimp, but tagging individuals for tracking pedigree is time-consuming, labor-intensive, and would cause inaccurate kinship. Molecular markers are effective in solving this problem. In this study, we developed 37 SNP markers derived from transcriptome sequences and performed analysis on genetic diversity, family clustering, and parentage for 22 families of Litopenaeus vannamei. The results provided examples for the application of SNP markers in selective breeding of shrimp. The results showed that the average expected heterozygosity (He) and observed heterozygosity (Ho) for 37 SNP loci were 0.38 and 0.34, respectively. Further, the average polymorphism information content (PIC) was 0.30, which means moderate polymorphism (0.25 |
