| 崔爱君,徐永江,王滨,姜燕,柳学周.黄条鰤生长性状全基因组关联分析.渔业科学进展,2021,42(2):71-78 |
| 黄条鰤生长性状全基因组关联分析 |
| Genome-wide association analysis of growth traits in yellowtail kingfish (Seriola lalandi) |
| 投稿时间：2020-02-05 修订日期：2020-03-02 |
| DOI：10.19663/j.issn2095-9869.20200205002 |
| 中文关键词: 黄条鰤 生长性状 全基因组关联分析 2b-RAD 简化基因组 |
| 英文关键词: Seriola lalandi Growth trait Genome-wide association study (GWAS) 2b-RAD Simplified genome |
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| 中文摘要: |
| 利用2b-RAD技术对119尾黄条鰤(Seriola lalandi)个体进行测序，共获得黄条鰤SNP分子标记26665个，对黄条鰤个体的体质量和全长这2个重要生长性状进行全基因组关联分析，筛选与体质量和全长性状相关的SNP位点和候选基因。结果显示，黄条鰤体质量性状中共筛选到17个体质量显著关联的SNP位点，找到17个可能的候选基因，全长性状共筛选到12个潜在显著关联位点，找到12个可能的候选基因。利用KEGG数据库对可能的候选基因进行Pathway分析，得知候选基因主要参与了细胞或组织生长发育相关的代谢通路调控过程，可能是影响黄条鰤生长性状密切相关的重要候选SNP位点和功能基因，结果可为今后黄条鰤种质资源可持续利用和育种提供遗传信息资料积累。 |
| 英文摘要: |
| The genetic resources available for the commercially important pelagic yellowtail kingfish (Seriola lalandi) are relative sparse. 2b-RAD simplified genome sequencing technology was applied to screen single nucleotide polymorphisms (SNPs) in yellowtail kingfish, and a total of 26,665 SNPs were obtained. A genome-wide association study was carried out to detect body weight- and total length-associated SNPs in 119 individuals from the yellowtail kingfish population in the Yellow Sea. The results showed that 17 SNPs associated with body weight and with potential genome-wide significance were found. Genes in the candidate regions with 1 Mb windows were screened, and 17 candidate genes were obtained. A total of 12 SNPs associated with total length and with potential genome-wide significance were identified, and 12 candidate genes were found. For these candidate genes, KEGG pathway analysis showed that they are mainly involved in the metabolic regulation pathway of growth and development in other vertebrates, which may be important candidate SNP loci and functional genes closely related to the growth traits of yellowtail kingfish. The present results could provide genetic information for the sustainable utilization of germplasm resources and genetic breeding of yellowtail kingfish in the in the future. |
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