| 李凤辉,边力,史会来,周丽青,陈四清,葛建龙,刘长琳,卢斌,常青.真蛸染色体核型分析.渔业科学进展,2021,42(5):132-138 |
| 真蛸染色体核型分析 |
| Karyotype analysis of the East Asian common octopus, Octopus sinensis |
| 投稿时间：2020-03-24 修订日期：2020-04-11 |
| DOI：10.19663/j.issn2095-9869.20200324001 |
| 中文关键词: 真蛸 染色体 核型 |
| 英文关键词: Octopus sinensis Chromosome Karyotype |
| 基金项目: |
|
| 摘要点击次数: 1614 |
| 全文下载次数: 1280 |
| 中文摘要: |
| 以东海沿海捕获的野生真蛸(Octopus sinensis)为实验材料，用含秋水仙素终浓度为0.005%的海水活体暂养的方法，取鳃和肾脏细胞，利用热滴片法制备染色体标本，对其核型进行分析。结果显示，真蛸的二倍体染色体条数为60条，核型公式为2n=14 m+26 sm+12 st+8 t，其染色体臂数(NF)为100。未发现带有随体和次溢痕的染色体和异型染色体，推断真蛸可能为常染色体性别决定类型。本研究可为蛸属的细胞遗传学研究及真蛸种质鉴定和资源保护提供基础资料和参考依据。 |
| 英文摘要: |
| Comparative studies of the karyotypes of closely related species may aid our understanding of the mechanisms of chromosomal evolution relevant to speciation. It may also help to clarify phylogenetic relationships. In the present study, we analyzed the karyotypes of ten wild immature East Asian common octopus (Octopus sinensis; 5 male and 5 female) from the coastal regions of the East China Sea. O. sinensis were held in seawater containing 0.005% colchicine for 2 h, and then the gill and kidney were collected for chromosome sample preparation via the hot-dropping method. The results showed that the diploid chromosomal number of O. sinensis is 60, and the karyotype formula is 2n = 14m + 26sm + 12st + 8t, NF = 100. No satellite, secondary constriction, or heteromorphic chromosomes of sex were observed. Therefore, we speculate that the sex determination mechanism of O. sinensis is autosomal. This study provides basic data for cytogenetic studies of Octopus and serves as a valuable reference for O. sinensis germplasm identification and conservation. |
| 附件 |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
