| 王国浩,董宣,王一婷,郭晓萌,王德浩,孟凡增,黄倢.患铁虾综合征罗氏沼虾眼柄转录组研究.渔业科学进展,2022,43(3):64-74 |
| Since 2010, iron prawn syndrome (IPS), characterized by sexual precocity and slow growth, has seriously affected the development of Macrobrachium rosenbergii aquaculture. To investigate the molecular mechanism of IPS, we performed transcriptome sequencing of M. rosenbergii with and without IPS using next-generation sequencing technology. This resulted in a total of 56.42 G of high-quality data being obtained. A total of 221 901 unigenes were obtained, and the length range was 201~30 985 bp. The average length was 1572 bp. The length of N50 was 2867 bp, and the length of N90 was 646 bp. Sequence alignment and functional annotation of the unigenes were conducted in the Nr, Nt, Swissprot, KEGG, KOG, GO, and PFAM databases. A total of 103 570 unigenes were annotated, and the GO database had the most annotated unigenes. The differential expression analysis revealed that 2003 genes, including 1209 upregulated genes and 794 downregulated genes, were differentially expressed in the eyestalks of M. rosenbergii with IPS. Consequently, 516 genes were mapped into 242 KEGG pathways, and the number of differentially expressed genes in translation, signal transduction, and the immune system was the largest. Many reproduction-related pathways were identified, including prolactin, estrogen, insulin, gonadotropin-releasing hormone (GnRH), glucagon, oxytocin, glutamatergic synapses, serotonergic synapses, and other metabolic processes related to reproductive regulation. In addition, some differentially expressed genes that have been proven to play an essential role in the immune response were upregulated in the eyestalks of M. rosenbergii with IPS, such as vascular endothelial growth factor receptor 1, serine proteinase inhibitor 6, C-type lectin, arylsulfatase B, prophenoloxidase-activating enzyme 2a, cathepsin B, cathepsin L, and crustin 4. At the same time, several immune-related pathways, such as lysosome, phagosome, antigen processing and presentation, apoptosis, endocytosis, and many others, were annotated. These results support the conclusion from the recent study that a pathogen is associated with IPS in M. rosenbergii. This study provides a foundation for understanding the causes and molecular mechanisms of IPS in M. rosenbergii. |
