|
| 胶州湾滩涂生计渔业渔获物“蚝艮”的分类鉴定及其分布记录* |
| Species identification of the subsistence fishery catch “Hao Gen” fish from the tidal flats of Jiaozhou Bay |
| 投稿时间：2024-09-06 修订日期：2024-10-08 |
| DOI： |
| 中文关键词: “蚝艮” 胶州湾 生计渔业 DNA条形码 物种鉴定 长体刺虾虎鱼 |
| 英文关键词: “Hao Gen” fish Jiaozhou Bay subsistence fishery DNA barcoding species identification Acanthogobius elongatus |
| 基金项目:国家现代农业产业技术体系资助项目(CARS-47-G31); 青岛市科技惠民示范专项(24-1-8-xdny-3-nsh); 中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022022024) |
|
| 摘要点击次数: 135 |
| 全文下载次数: 0 |
| 中文摘要: |
| 为明确胶州湾滩涂生计渔业渔获物“蚝艮”的种类及其分布信息，本研究利用形态学方法和DNA条形码技术对该物种开展了分类鉴定。在胶州湾北部滩涂生计渔业的传统作业区共采集“蚝艮”样品10尾。形态学研究选取了7个可数性状和7个可量性状对样品进行定量分析，并通过与历史文献记录比较，初步确定该物种为长体刺虾虎鱼[Acanthogobius elongatus (Fang, 1942)]。测序获取样品DNA条形码序列10条，在GenBank数据库中进行相似性比对，结合已报道的8种虾虎鱼类DNA条形码进行分析，构建系统进化关系，进一步确定样品的种类及分类地位。结果显示：“蚝艮”的DNA 条形码与已报道的长体刺虾虎鱼序列具有高度的相似性，二者的遗传距离为种内差异水平；在系统发育树中，“蚝艮”与长体刺虾虎鱼聚为一支，支持将“蚝艮”鉴定为长体刺虾虎鱼。本研究首次对“蚝艮”实施了科学鉴定，并记录了其在胶州湾的分布，解决了该生计渔业渔获物长期以来种类不明的问题。研究结果将丰富胶州湾鱼类多样性认知，并为胶州湾渔业资源的养护和生计渔业管理措施的制定提供科学基础。 |
| 英文摘要: |
| To clarify the species identify and distribution information of the “Hao Gen” fish, a kind of fish catch from the subsistence fishery in tidal flat of Jiaozhou Bay of Yellow Sea, this study conducted taxonomic identification using morphological methods and DNA barcoding technology. A total of 10 “Hao Gen” fish samples were collected from a traditional fishing area of the tidal flat in the northern part of Jiaozhou Bay. In morphological study, seven countable traits and seven measurable characteristics were selected and used for the quantitative analysis of samples, and by comparing with historical literature records, the species was preliminarily identified as Acanthogobius elongatus (Fang, 1942). Meanwhile, we examined the DNA barcode fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene of all samples. BLAST searches were performed to identify similar sequences in GenBank. Homologous sequences from closely-related species were also included in the comparative analysis and species identification. Phylogenetic relationships among 8 species of the family Gobiidae were reconstructed using Neighbour joining (NJ) based Kimura-2 parameter (K2P) net genetic distance. The results indicated that all DNA barcode sequences of the “Hao Gen” samples showed a high degree of similarity to the homologous sequences of the species Acanthogobius elongatus, and the genetic distance between them was at the intraspecific level. In the phylogenetic tree, all the “Hao Gen” samples clustered with DNA barcode sequences of Acanthogobius elongatus which had been published in GenBank. It was shown that the results of morphological and DNA barcoding analysis were consistent, indicating that the “Hao Gen” samples could be identified as Acanthogobius elongatus. In this study, the effective identification of “Hao Gen” fish and the first record of the species in Jiaozhou Bay addressing the long-standing issue of the lack of scientific understanding for the subsistence fishery catch species. These findings will enrich the knowledge of fish diversity in Jiaozhou Bay and provide a scientific basis for the conservation of fishery resources and the improvement of fisheries management in the area. |
| 附件 |
|
View Fulltext
查看/发表评论 下载PDF阅读器 |
| 关闭 |
