文章摘要
利用微卫星标记分析军曹鱼养殖群体的遗传多样性
Genetic diversity in five cultured population of cobia (Rachycentron canadum) using microsatellite marker
投稿时间:2019-06-17  修订日期:2019-08-20
DOI:
中文关键词: 军曹鱼  养殖群体  微卫星  遗传多样性
英文关键词: Rachycentron canadum, Culture population, Microsatellite, Genetic diversity
基金项目:国家海水鱼产业技术体系-军曹鱼种质资源与品种改良岗位
作者单位E-mail
李伟强 广东海洋大学水产学院 lwqclj@126.com 
陈刚 广东海洋大学水产学院 cheng@gdou.edu.cn 
马骞 广东海洋大学水产学院  
黄建盛 广东海洋大学水产学院  
施钢 广东海洋大学水产学院  
潘传豪 广东海洋大学水产学院  
周晖 广东海洋大学水产学院  
谢瑞涛 广东海洋大学水产学院  
张健东 广东海洋大学水产学院  
汤保贵 广东海洋大学水产学院  
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中文摘要:
      利用12个微卫星标记对北海(BH)、陵水(LS)、硇洲(NZ)、徐闻(XW)和三亚(SY)5个军曹鱼养殖群体进行遗传多样性分析。结果显示,12对微卫星引物在5个军曹鱼养殖群体中共检测到129个等位基因。各养殖群体的平均等位基因数为3.833~6.750,平均有效等位基因数为2.525~3.645,平均观测杂合度和平均期望杂合度为0.481~0.635和0.530~0.681,平均多态信息含量为0.463~0.630;哈迪-温伯格平衡检测结果显示,各群体在多个微卫星位点上均显著偏离平衡(P<0.05);军曹鱼养殖群体间的遗传分化指数(Fst)为0.055~0.150,遗传距离(D)为0.240~0.635,其中BH和NZ的Fst最大(0.150),D最远(0.635);AMOVA分析结果表明,军曹鱼养殖群体84%遗传变异来自于个体之间;基于Nei’s遗传距离构建的UPGMA系统进化树显示,BH和SY聚为一支,LS和XW聚为一支,两支聚为一支后与NZ聚为一支。研究结果将为军曹鱼种质资源保护和改良等提供科学的数据参考。
英文摘要:
      Cobia, Rachycentron canadum, the only species in the family Rachycentridae, is a candidate species for cage culture in tropical and subtropical waters. Taiwan was the first to cage culture of cobia in the early 1990s, then culture of cobia has also been developed in Southeast Asia and other areas. With the development of marine fisheries and aquaculture, understanding the genetic diversity of cultured population is important for the sustainable development of aquaculture species. In the present study, twelve polymorphic microsatellite loci were selected to investigate and assess the level of genetic diversity in five cultured population of cobia from Beihai (BH), Lingshui (LS), Naozhou (NZ), Xuwen (XW) and Sanya (SY). As a result, a total of 129 alleles were detected in five cultured populations. The mean number of alleles was between 3.833 and 6.750, the mean number of effective alleles was from 2.525 to 3.645, the mean of observed heterozygosity and expected heterozygosity was between 0.481 and 0.635 of between 0.530 and 0.681, the mean polymorphism information content was from 0.463 to 0.635. The population deviated significantly from Hardy-Weinberg equilibrium at multiple microsatellite loci (P < 0.05). The analysis of genetic differentiation indicated that the Fst range was from 0.055 to 0.150 and genetic distance (D) range was from 0.240 to 0.635. BH and NZ had the highest Fst (0.150), and the highest D (0.635). The results of AMOVA analysis showed that 84% of the genetic variations were within cultured populations, and 16% of the genetic variations were among cultured populations. The UPGMA phylogenetic analysis based on Nei's genetic distance showed that one cluster comprised of BH and SY and the other cluster comprised of LS and XW formed a branch, which was then clustered with NZ. These results in the present study would provide scientific data for further protection and improvement of the germplasm resources of cobia.
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