文章摘要
对虾养殖池副溶血弧菌的分离鉴定及其耐药特征、毒力基因分析
Isolation and Identification of Vibrio parahaemolyticus From Shrimp Culture Ponds and Analysis of its Drug Resistance and Virulence Genes
投稿时间:2019-08-20  修订日期:2019-09-23
DOI:
中文关键词: 副溶血弧菌  耐药性  毒力基因  对虾养殖池
英文关键词: Vibrio parahaemolyticus  Antibiotic resistance  Virulence gene  Shrimp culture ponds
基金项目:现代农业产业技术体系专项,泰山产业领军人才工程项目
作者单位E-mail
李翠苹 上海海洋大学水产与生命学院 上海 lcp1201@163.com 
翟倩倩 农村农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛  
王想 海水养殖教育部重点实验室 中国海洋大学 青岛  
李健 农村农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食品产出过程功能实验室 lijian@ysfri.ac.cn 
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中文摘要:
      为了解对虾养殖池中副溶血弧菌的耐药性和毒力基因的携带情况,2018 年从山东4个地区对虾养殖池收集分离副溶血弧菌,采用Kirby-Bauer纸片法检测其对 12 种抗生素的耐药性,用 PCR方法检测其携带耐热直接溶血素基因(tdh)和耐热相关溶血素基因(trh)的情况。从对虾养殖池共分离副溶血弧菌 50株。药敏试验结果显示,副溶血弧菌对庆大霉素、硫酸新霉素和氨苄西林的耐药情况最为严重,耐药率分别 98 % 、90 % 和86 %,对氟苯尼考,氯霉素,头孢他啶等敏感性较高,耐药率分别为 10 %、10 %、20 %。且88 % 的菌株具有多重耐药性。毒力基因检测结果显示,所有菌株均不携 tdh 基因,4 % 的菌株表现为 trh 阳性。本研究提示对虾养殖水环境中的副溶血弧菌对抗生素的耐药性较为严重,应加强副溶血弧菌的病原学监测,在养殖过程中合理使用抗生素,以实现水产养殖业健康发展。
英文摘要:
      In order to understand the drug resistance and virulence genes of Vibrio parahaemolyticus in shrimp culture ponds, we collected and isolated V. parahaemolyticus from shrimp culture ponds in four different areas of Shandong Province in 2018. Kirby-Bauer disk method was used to detect its resistance to 12 antibiotics, and PCR method was used to detect its carrying heat-resistant direct hemolysin gene (tdh) and heat-resistant related hemolysin gene (trh). A total of 50 vibrio parahaemolyticus strains were isolated from Shrimp culture ponds.Drug susceptibility test showed that the resistance of V. parahaemolyticus to gentamicin, neomycin sulfate and ampicillin was the most serious. The resistance rates were 98 %, 90 % and 86 %, respectively. The susceptibility rates to florfenicol, chloramphenicol and ceftazidime were higher, and the resistance rates were 10 %, 10 % and 20 %, respectively. 88 % of the strains showed multiple drug resistance.The results of virulence gene test showed that all strains did not carry tdh gene, and 4 % strains were trh positive. This study suggests that the antibiotic resistance of V. parahaemolyticus in shrimp aquaculture environment is serious. Etiological surveillance of V. parahaemolyticus should be strengthened and antibiotics should be reasonably used in the aquaculture process to realize the healthy development of aquaculture industry
相关附件:   附件1  修改说明  序列  部分副溶血弧菌trh基因扩增电泳图谱  附件1复审意见回复  附件2图1矢量图  附件1
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