文章摘要
低氧-复氧对脊尾白虾呼吸代谢和抗氧化酶活力的影响
Effects of Hypoxia and Reoxygenation on Activities of Respiratory Metabolism Enzyme and Antioxidant Enzyme of Exopalaemon carinicauda
投稿时间:2020-04-24  修订日期:2020-05-21
DOI:
中文关键词: 脊尾白虾  低氧  呼吸代谢  抗氧化能力
英文关键词: Exopalaemon carinicauda  Hypoxia  Respiratory Metabolism  Antioxidant capacity
基金项目:
作者单位E-mail
王 盼 上海海洋大学 水产科学国家级实验教学示范中心 w18738397563@163.com 
史文军# 江苏省海洋水产研究所  
万夕和① 江苏省海洋水产研究所 wxh1708@163.com 
沈 辉 江苏省海洋水产研究所  
沙士兵 江苏省海洋水产研究所  
黎 慧 江苏省海洋水产研究所  
王李宝 江苏省海洋水产研究所  
孙瑞健 江苏省海洋水产研究所  
乔 毅 江苏省海洋水产研究所  
蒋 葛 江苏省海洋水产研究所  
成 婕 江苏省海洋水产研究所  
吴旭干 上海海洋大学 水产科学国家级实验教学示范中心  
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中文摘要:
      为了研究低氧-复氧对脊尾白虾(Exopalaemon carinicauda)呼吸代谢和抗氧化能力的影响,以静室呼吸耗氧—复氧为实验组,溶氧为7.97±0.22 mg/L作为对照组,分别于实验开始的0、1、2、4、5 h及复氧1、4、8 h对水体和虾组织进行取样,检测各时间点水体中溶解氧浓度和虾组织主要呼吸代谢及抗氧化酶的活力。结果显示:(1)随着静室呼吸耗氧时间的延长,实验组水体溶解氧含量不断降低,且显著低于对照组(P<0.05),对照组水体溶解氧保持平稳水平;复氧后,实验组水体溶解氧含量迅速恢复到对照水平。(2)随着时间的延长,脊尾白虾鳃、肝胰腺和肌肉组织中呼吸代谢酶类细胞色素C氧化酶(CCO)、琥珀酸脱氢酶(SDH)活力均不断降低,乳酸脱氢酶(LDH)、延胡索酸还原酶(FRD)活力均不断升高,5 h CCO、SDH活力下降到最低,且显著低于对照组(P<0.05),LDH、FRD活力达到最大值,且显著高于对照组(P<0.05);复氧后,3种组织CCO、SDH活力又逐渐升高,但肌肉组织CCO活力在复氧8 h时显著低于对照组(P<0.05);LDH、FRD活力复氧1 h时在3种组织中均显著下降(P<0.05),继续复氧处理时逐渐恢复至对照组水平。 (3)随着时间的延长,脊尾白虾鳃、肝胰腺和肌肉组织中抗氧化酶类超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)和谷胱甘肽S转移酶(GST)活力均呈现先升高后降低的变化趋势,4 h到达最大值且显著高于对照组;但过氧化物酶(POD)活力在肝胰腺组织中逐渐降低,而在鳃和肌肉组织呈波动性变化趋势;复氧后,鳃、肝胰腺和肌肉组织中SOD、CAT活力先升高后降低,在复氧8 h时与对照组均无显著性差异(P>0.05);复氧后,3种组织中GPX和GST活力均恢复至对照组水平,但鳃和肌肉组织POD在复氧8 h时均显著低于对照组(P<0.05)。综上所述,随着水体溶解氧含量的不断降低,脊尾白虾有氧代谢水平逐渐降低,无氧代谢能力逐渐升高,复氧后,有氧代谢能力又逐渐恢复;SOD、CAT、POD、GPX和GST等抗氧化酶活力的变化可能在脊尾白虾应对环境低氧及在复氧过程中产生的氧化损伤发挥着重要作用。
英文摘要:
      To study the effects of hypoxia and reoxygenation on respiratory metabolism and antioxidant capacity of Exopalaemon carinicauda. In the present study, Oxygen consumption -reoxygenation in respiratory chamber were the experimental group and dissolved oxygen (7.97±0.19) mg/L were used as the control group. Samples of water and shrimp tissue were taken at 0, 1, 2, 4, 5h of hypoxia and 1, 4, 8h of reoxygenation after the beginning of the experiment. Further, we detected the dissolved oxygen concentration of water and the activities of main respiratory metabolism enzyme and antioxidant enzyme of shrimp tissue in different time points. The results indicated that: (1) with prolong of oxygen consumption time in respiratory chamber, the dissolved oxygen concentration in the experimental group decreased continuously and was significantly lower than those in the control group (P<0.05). The dissolved oxygen in the control group remained stable level. After reoxygenation, the dissolved oxygen concentration in the experimental group quickly returned to the control level. (2) With prolong of the time, Cytochrome c oxidase (CCO) and succinodehydrogenase (SDH) activities of the gill, hepatopancreas and muscle from the experimental group decreased continuously, lactic dehydrogenase (LDH) and fumaric reductase (FRD) activities increased continuously. The activities of CCO and SDH showed a minimum value were significantly lower than those of the control group at 5 h (P<0.05), the activities of LDH and FRD showed a maximum value were significantly higher than those of the control group at 5 h (P<0.05). With the increase of recovery time, CCO and SDH activities gradually increased in three organizations, but CCO activity in muscle was significantly lower than those in the control group at reoxygenation for 8 h (P<0.05). LDH and FRD activity decreased significantly in all three tissues at 1 h of reoxygenation (P<0.05), and gradually returned to the level of the control group with the prolong of recovery time. (3) With prolong of the time, the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and Glutathione s-transferase (GST) in gill, hepatopancreas and muscle all showed a trend of first increase and then decrease, and the activities of the four enzymes reached a maximum value were significantly higher than those of the control group at 4 h (P<0.05). The activity of peroxidase (POD) decreased continuously in hepatopancreas, was fluctuated in gill and muscle. After reoxygenation, the activity of SOD and CAT in gill, hepatopancreas and muscle all showed a trend of first increase and then decrease, and without significant difference between the control group in 8 h(P>0.05). With the increase of recovery time, the activity of GPX and GST of the three tissues returned to the level of the control group, but the activity of POD in gill and muscle were significantly lower than those in the control group at 8h of reoxygenation. Overall, with the continuous decrease of dissolved oxygen concentration, the aerobic metabolic level of the Exopalaemon carinicauda decreased gradually, and the anaerobic metabolic capacity gradually increased, after reoxygenation, the aerobic metabolic capacity gradually recovered. Changes in the activity of SOD, CAT, POD, GPX and GST may play an important role in responding to oxidative damage during hypoxia and reoxygenation.
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