文章摘要
缢蛏急性高温胁迫应答主要候选基因的表达特征分析
Tab. 1 Primers information of genes in qRT- PCR
投稿时间:2020-12-23  修订日期:2021-01-26
DOI:
中文关键词: 缢蛏  高温胁迫  qRT-PCR  基因表达
英文关键词: Sinonovacula constricta  thermal stress  qRT-PCR  gene expression
基金项目:国家重点研发计划“蓝色粮仓科技创新”专项课题(2018YFD0901405);浙江省农业新品种选育重大科技专项课题(2016C02055-9);宁波市“科技创新2025”重大专项(2019B10005);国家海洋水产种质资源库项目
作者单位E-mail
孔祥辉 上海海洋大学 水产科学国家级实验教学示范中心 上海
浙江万里学院 浙江水产种质资源高效利用技术研究重点实验室 浙江 宁波
浙江万里学院 宁海海洋生物种业研究院 浙江 宁海 
kongxianghui416@126.com 
王莎莎 浙江万里学院 浙江水产种质资源高效利用技术研究重点实验室 浙江 宁波  
董迎辉 浙江万里学院 浙江水产种质资源高效利用技术研究重点实验室 浙江 宁波 dongyinghui118@126.com 
任建峰 上海海洋大学 水产科学国家级实验教学示范中心 上海  
林志华 浙江万里学院 浙江水产种质资源高效利用技术研究重点实验室 浙江 宁波  
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中文摘要:
      缢蛏(Sinonovacula constricta)为广温性贝类,自身存在特殊的防御机制以适应外界温度胁迫。为研究高温胁迫对缢蛏热应激相关基因表达的影响,用qRT-PCR技术对转录组中筛选的3类11个高温应答候选基因,即分子伴侣类基因(HSP70、HSF1、GRP94、BAG3、PDIA6、CALR)、代谢与免疫应答类基因(MDH、CTL、CTSL)和细胞凋亡类基因(Cascrap-3、p53),在不同温度(30℃、32℃、34℃)胁迫下鳃和肝胰腺中的表达特征进行了分析。结果显示,分子伴侣类基因受温度影响最为显著,从胁迫4 h时表达量开始显著升高,与温度呈正相关,且鳃较肝胰腺更早做出响应;代谢与免疫应答和细胞凋亡类基因在鳃、肝胰腺中的表达均随胁迫时间延长呈先升高后下降趋势;免疫应答类基因在肝胰腺中响应更显著。综上所述,高温胁迫下缢蛏维持基本生命稳态与这3类基因的调控密切相关。本研究为进一步探索贝类高温响应分子机制奠定理论基础,也为耐高温缢蛏新品种的分子标记辅助选育提供候选基因。
英文摘要:
      The razor clam, Sinonovacula constricta, as eurythermogenic bivalve, has special adaptive mechanisms to defense the environment stress because of its special lifestyle and limited range of activities. To understand the expression characteristics of genes involved in different metabolic processes, qRT-PCR technology was used to analyze the expression levels of three types temperature response candidate genesS(molecular chaperone genes, metabolic and immune related genes and apoptosis genes) based on transcriptomic analysis in gills and hepatopancreas under different acute high temperature conditions (30℃, 32℃, 34℃). The results showed that the expression of molecular chaperone genes was significantly up-regulated at 4 h under thermal stress, and was positively correlated with temperature. Meanwhile, gills responded earlier than the hepatopancreas. The mRNA expression of metabolic and immune responses and apoptotic genes exhibited an increase and then decrease in both tissues with the extension of stress time. In conclusion, the regulation of these genes was played significant roles to maintain basic homeostasis in S. constricta under heat stress. The immune response genes were more significant expression in hepatopancreas. This study provides a theoretical basis for further exploring the molecular mechanism of high temperature response of S. constricta andSalso provides candidate genes for molecular marker assisted breeding in this species under thermal stress.
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