文章摘要
红鳍东方鲀(Takifugu rubripes)低温胁迫应答主效QTL候选基因的表达特征分析
Expression Characteristics Analysis of Major QTL Candidate Genes Responding to Low Temperature Stress in Takifugu rubripes
投稿时间:2022-06-08  修订日期:2022-07-19
DOI:
中文关键词: 红鳍东方鲀  pde10a基因  tacc2基因  unc5b基因  exoc4基因  arhgap44a基因  fsip1基因  低温胁迫
英文关键词: Takifugu rubripes  tacc2  fsip1  exoc4  arhgap44a  pde10a  unc5b  low temperature stress
基金项目:国家重点研发计划子课题(2018YFD0900301-12)、国家重点研发计划子课题 (2018YFD0900102-5)、国家自然科学基金项目(32002362)、山东省自然科学基金(ZR2019BC089)和中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2020TD25)共同资助
作者单位邮编
朱理光 大连海洋大学水产与生命学院
中国水产科学研究院黄海水产研究所山东省海洋渔业生物技术与遗传育种重点实验室青岛市海水鱼类种子工程与生物技术重点实验室
青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室 
266071
刘志峰 中国水产科学研究院黄海水产研究所山东省海洋渔业生物技术与遗传育种重点实验室青岛市海水鱼类种子工程与生物技术重点实验室
青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室 
马爱军 中国水产科学研究院黄海水产研究所山东省海洋渔业生物技术与遗传育种重点实验室青岛市海水鱼类种子工程与生物技术重点实验室
青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室 
266071
王新安 中国水产科学研究院黄海水产研究所山东省海洋渔业生物技术与遗传育种重点实验室青岛市海水鱼类种子工程与生物技术重点实验室
青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室 
孙志宾 中国水产科学研究院黄海水产研究所山东省海洋渔业生物技术与遗传育种重点实验室青岛市海水鱼类种子工程与生物技术重点实验室
青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室 
常浩文 中国水产科学研究院黄海水产研究所山东省海洋渔业生物技术与遗传育种重点实验室青岛市海水鱼类种子工程与生物技术重点实验室
青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室 
刘圣聪 唐山牧海水产养殖有限公司 
包玉龙 唐山牧海水产养殖有限公司 
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中文摘要:
      红鳍东方鲀为暖温性鱼类,冬季海水的低温会对其生存造成很大影响,所以选育具有抗寒性状的品系对产业发展具有重要意义。本研究在利用QTL定位分析筛选出6个与红鳍东方鲀耐低温相关候选基因(tacc2、fsip1、exoc4、arhgap44a、pde10a和unc5b)的基础上,通过Real-time PCR检测这6个基因在低温胁迫下肝脏、心脏、肾脏中表达量的变化。实验用鱼为课题组建立同一家系的8月龄鱼,共设置三个温度梯度,包括8℃,13℃和18℃,8℃和13℃为低温组,18℃为对照组。实验结果显示,6个基因在不同温度下的3个组织中均有不同程度的表达。其中pde10a基因在3个组织中的表达都呈现先升高后下降的趋势。tacc2和exoc4基因在肝脏,肾脏以及心脏8℃组中的表达分别呈现先下降再趋于稳定,先升高再趋于稳定和先上升后下降的趋势。unc5b基因在肝脏和心脏中的表达量较低,肾脏低温组在实验前期呈现高表达。arhgap44a基因在肝脏中的表达呈现上升趋势;在心脏和肾脏中整体表达无明显变化。fsip1基因在肝脏中的表达呈现下降趋势;在心脏和肾脏中的表达呈现先上升后下降趋势。这6个基因在红鳍东方鲀组织中都随着时间和温度变化具有差异性表达,在低温胁迫下呈现积极响应,表明这6个QTL候选基因在红鳍东方鲀低温适应中具有潜在的重要作用。虽然低温是限制红鳍东方鲀产业发展的一个很重要的因素,但对红鳍东方鲀耐低温相关的研究很少有报道,通过本研究可为红鳍东方鲀耐低温相关信号通路研究以及耐低温品种选育提供理论依据。
英文摘要:
      Takifugu rubripes are warm temperate fish, and the low temperature of seawater in winter will have a great impact on its survival, so breeding new cold tolerant varieties is of great significance to industrial development. In this study, based on the 6 QTL candidate genes (tacc2、fsip1、exoc4、arhgap44a、pde10a and unc5b) related to low temperature tolerance of Takifugu rubripes , the expression changes of these six genes in liver, heart and kidney were detected by real-time quantitative PCR under low temperature stress. The experiment fish was the 8-month-old fish from same family established by our research group, setting three temperature gradients, including 8℃, 13℃ and 18℃, 8℃ and 13℃ for low temperature group, 18℃ for control group. The results showed that the six genes were expressed at different levels in three tissues at different temperatures. The relative expression of pde10a firstly increased and then decreased in three tissues. The relative expression of tacc2 and exoc4 genes in liver, kidney and heart at 8℃ first decreased and then tended to be stable, first increasing and then stabilizing, first increasing and then decreasing respectively. The relative expression of unc5b gene was low in liver and heart, but in kidney showed high expression in the second week in low temperature group. The relative expression of arhgap44a gene in liver showed an upward trend, and there was no significant difference in overall expression level in kidney and heart. The relative expression of fsip1 gene in liver showed a downward trend, in the heart and kidney showed a trend of first increasing and then decreasing. The results showed that the six genes were differentially expressed in Takifugu rubripes tissues with time and temperature changes, and showed positive responses to low temperature stress. In conclusion, these six QTL candidate genes play a potentially important role in low temperature adaptation of Takifugu rubripes. Although low temperature is a very important factor limiting the development of the industry of Takifugu rubripes, there are few reports on its related research. This study provides a theoretical basis for the study of signal pathways related to low temperature tolerance of Takifugu rubripes and the breeding of low temperature tolerance varieties.
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