文章摘要
镉对绿鳍马面鲀幼鱼急性毒性、肝脏抗氧化能力及组织结构的影响
Acute effects of cadmium on juvenile, antioxidant enzyme activities in liver and histological structure of gill and liver of Thamnaconus septentrionalis
投稿时间:2022-06-27  修订日期:2022-07-06
DOI:
中文关键词: 绿鳍马面鲀    组织结构  抗氧化酶
英文关键词: Thamnaconus septentrionalis  Cadmium  Biological tissue  antioxidant enzyme
基金项目:
作者单位邮编
王晓然 上海海洋大学水产与生命学院 266071
边 力 中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 
胡 琼 中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 
秦 搏 中国水产科学研究院东海水产研究所
中国水产科学研究院东海水产研究所 
常 青 中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 
英 娜 中国水产科学研究院东海水产研究所
中国水产科学研究院东海水产研究所 
吴艳庆 中国水产科学研究院东海水产研究所
中国水产科学研究院东海水产研究所 
杨立国 中国水产科学研究院东海水产研究所
中国水产科学研究院东海水产研究所 
陈四清 中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 266071
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中文摘要:
      试验采用静水生物测试法测定了镉(Cd2+)对绿鳍马面鲀(Thamnaconus septentrionalis)幼鱼的急性毒性;根据预实验结果,设定8.19、9.18、10.30、11.56 mg/L 4个Cd2+浓度梯度进行急性毒性试验,根据急性毒性试验结果,设定1.84、2.76、3.68和4.60 mg/L 4个不同浓度Cd2+急性暴露实验,分别在6h、12h、24h、48h、72h、96h检测肝脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽抗氧化酶(GSH-PX)活性及丙二醛(MDA)含量和肝脏、鳃组织结构的变化。结果表明,随着Cd2+浓度增加,急性毒性效应逐渐增强,24h、48h、72h、96h LC50分别为11.47、10.82、9.84、9.19 mg/L ,Cd2+对绿鳍马面鲀幼鱼的96h安全浓度为0.92 mg/L;6h,各浓度组SOD和CAT活性与对照组相比有极显著升高,6~48h,SOD、CAT、GSH-PX活性均呈先上升后下降的趋势,48~96h,各浓度组酶活性均下降,且时间越长,浓度越大,活性越低。与对照组相比,MDA含量整体趋势呈先降低后增加,12~48h,1.84 mg/L、2.76 mg/L组MDA含量有波动,3.68 mg/L、4.60 mg/L组MDA含量与时间和浓度成正比。24h时,1.84 mg/L组肝脏组织未见明显变化,2.76 mg/L、3.68 mg/L、4.60 mg/L组肝脏组织开始受到明显损伤,表现为细胞体积增大且形状不规则,细胞膜边界模糊,24h时1.84 mg/L、2.76 mg/L组鳃组织无显著变化,3.68 mg/L、4.60 mg/L组出现鳃小片弯曲,上皮细胞水肿膨大,相邻鳃小片相互粘连融合,无游离端,细胞坏死脱落等损伤现象。在安全浓度0.92 mg/L 内绿鳍马面鲀幼鱼可健康生长,SOD、CAT和GSH-PX活性变化及MDA含量反映了绿鳍马面鲀幼鱼受损害程度,可作为安全性风险评价的参考依据。
英文摘要:
      The acute toxicity of cadmium (Cd2+) to juvenile Thamnaconus septentrionalis was determined by hydrostatic biological assay. According to the pre-experiment results, four Cd2+ concentration gradient test groups (8.19, 9.18, 10.30, 11.56 mg/L) and one blank control group were selected to carry out acute toxicity test by equilogarithmic spacing method. The mortality, LC50 and safe concentration of juvenile fish at 24h, 48h, 72h and 96h were calculated. According to the experimental results of acute toxicity of Cd2+ to juvenile Thamnaconus septentrionalis, the concentration gradients were set as 1.84 mg/L, 2.76 mg/L, 3.68 mg/L and 4.60 mg/L at the safe concentration multiple. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione antioxidant enzyme (GSH-PX) and malondialdehyde (MDA) in liver and gill tissues were detected at 6h, 12h, 24h, 48h, 72h and 96h, respectively. The results showed that with the increase of Cd2+ concentration, the acute toxicity gradually increased, the LC50 of 24h, 48h, 72h and 96h were 11.47, 10.82, 9.84 and 9.19 mg/L, respectively, and the safe concentration of Cd2+ on juvenile Filefish was 0.92 mg/L at 96h. Within 6h, SOD activity in each concentration group was significantly higher than that in the control group (P<0.01); SOD activity first decreased and then increased within 6-24h. From 24h to 96h, SOD activity of all concentration groups showed a downward trend. SOD activity in 1.84 mg/L and 2.76 mg/L groups was always higher than that in control group within 96h. The enzyme activity of 3.68 mg/L and 4.60 mg/L groups decreased to that of the control group from 6 to 12h, and increased at 24h, and then remained in a downward trend and lower than that of the control group. From 6 to 12h, MDA content first decreased and then increased. At 24h, the enzyme content in each treatment group decreased, but it was still higher than that in the control group, and the difference was significant (P<0.05), 12 to 48h, 1.84 mg/L and 2.76 mg/L groups showed a trend of first decrease and then increase; Within 24 to 96h, 3.68 mg/L and 4.60 mg/L groups kept increasing, and were significantly higher than those in control group (P<0.01); At 6h, compared with the control group, CAT activity in all treatment groups was significantly increased; Compared with the control group, CAT activity in 3.68 mg/L and 4.60 mg/L groups was significantly inhibited at 48-96h. On the whole, the activity of GSH-PX in each treatment group showed a decreasing, increasing and decreasing trend within 6-96h. The activity of GSH-PX in 1.84 mg/L group was higher than that in control group within 96h; Compared with the control group, the activity of 2.76 mg/L and 3.68 mg/L groups decreased from 24 h to 96h, and the longer the stress time, the lower the activity. In the highest concentration 4.60 mg/L group, the enzyme activity increased first and then decreased, and the GSH-PX activity decreased to 300.12U at 96h, which was significantly different from that in the control group (P<0.01). In 1.84 mg/L group, there was no significant change in liver tissue within 72h, but in 1.84 mg/L group at 96h and 2.76 mg/L group at 24h, cell volume was slightly increased with irregular shape and some cell membrane boundary was blurred. At 48h-96h in 2.76 mg/L group, 24h-48h in 3.68 mg/L and 4.60 mg/L groups, the liver cells were disordered and scattered, and the structure of liver cells was mostly incomplete. After 72 to 96 hours in 3.68 mg/L group and 72 hours in 4.60 mg/L group, the abnormality of liver cells was significantly aggravated, including cell hypertrophy, large area disintegration, cytoplasm overflow and cell cavitation. At 96h in 4.60 mg/L group, the nucleus shrank, intracellular material gathered and large irregular blank appeared. In 1.84 mg/L group for 72h and 2.76 mg/L group for 24h, there was no significant change in gill tissue structure. In 1.84 mg/L group for 96h, 2.76 mg/L group for 48h and 4.60 mg/L group for 24h, the gill microplates were curved and club-like with mucus secreted around them, and the epithelial cells were edema and enlargement, and partial hyperplasia appeared. The distance between the adjacent lamella became smaller. In 2.76 mg/L group at 72h, 3.68 mg/L and 4.60 mg/L group at 24h to 48h, a small number of adjacent gill lamellae would fuse and form a small epithelial cell plate, with a large amount of mucus around, and the length of gill lamellae was significantly shortened. In the 2.76 mg/L group for 96h and 3.68 mg/L group for 72h, most of the basal lamellae fused together, and the epithelial cells of the gill lamellae were hyperplasia and swelling, and exfoliated epithelial cells were scattered around. At 96h in 3.68 mg/L group and 72-96h in 4.60 mg/L group, adjacent gill lamellae adhered and fused with each other without free end, and a large number of cells were necrotic and exfoliated.
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