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| 基于转录组学探究除虫脲暴露对鲤肝脏基因表达的影响* |
| Effect of diflubenzuron exposure on gene expression in Cyprinus carpio liver based on transcriptomicsCHEN Lu1,2 ,Yang Zhen2,Xu Jinhua2,QIAO Lu2,Song Jinlong2, |
| 投稿时间：2023-02-10 修订日期：2023-02-23 |
| DOI： |
| 中文关键词: 鲤 除虫脲 肝脏 转录组学 毒理 |
| 英文关键词: carp diflubenzuron liver transcriptomics toxicology |
| 基金项目:国家农产品质量安全风险评估专项项目（GJFP20210401）和虾蟹及淡水鱼质量安全风险评估与防控创新团队项目（2020TD75） |
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| 中文摘要: |
| 除虫脲 (DFB) 是一种常用的杀虫剂，其残留代谢物会威胁水生动物及其人体健康，为探究水产动物响应 DFB 胁迫的分子机制，本研究以鲤 (Cyprinus carpio) 为研究对象，选定 0.1 mg/L 和 1.0 mg/L 的药浴浓度对鲤进行 15 d 暴露实验。采用 RNA-seq 技术对肝脏开展转录组测序，以 P-adjust<0.05 和 |log2FC|≥1 为标准筛选差异表达基因 (DEGs) ，进行 GO 功能注释和 KEGG 富集分析等生物信息学分析。结果显示，0.1 mg/L 暴露浓度下有 2 406 个 DEGs 发生显著变化，1.0 mg/L 暴露浓度下有 2 688 个 DEGs 发生显著变化，两组间共表达的 DEGs 有 821 个。GO 分析结果表明，DFB 暴露组 DEGs 富集在生物过程、细胞组成和分子功能上。KEGG 富集分析表明，低浓度 DFB 暴露组 DEGs 显著富集到了异生物质的生物降解和代谢、脂质代谢、碳水化合物代谢、氨基酸代谢、信号分子与相互作用、内分泌系统、免疫系统等代谢通路；高浓度 DFB 暴露组 DEGs 除了富集到上述代谢通路外，还显著富集到了折叠、分类和降解、运输和分解代谢等代谢通路。结果表明，DFB 暴露对鲤造成异生物质的生物降解和代谢、脂质代谢、碳水化合物代谢、氨基酸代谢紊乱，并产生内质网应激、炎症反应和免疫毒性。本文从转录层面上解析了 DFB 暴露对鲤毒性作用的分子机制，为 DFB 在水产领域的限量标准制定和环境安全评价提供数据基础。 |
| 英文摘要: |
| As a benzoylurea insecticide, diflubenzuron (DFB) has been widely used in the prevention and control of diseases and insect pests in vegetables, fruits, grain cultivation and other fields in recent years. Because of its low acute toxicity, excellent biological activity and specific action mechanism, diflubenzuron has been widely used in aquaculture to kill bacteria and lice. With its large-scale production and widespread use, partial residue occurred in environmental media such as water, soil and atmosphere and caused direct or indirect chronic toxicity to aquatic organisms. The residual diflubenzuron entered the human body along with the food chain and posed a threat to human health when it reached a certain concentration through chronic exposure and long-term accumulation. At present, the research on DFB insecticide was limited to the usage and dosage, residual metabolism and its toxicological effects on the environment. There was no report on the effect of diflubenzuron on gene expression in carp liver in China. The purpose of this study was to explore the differential expression of genes in carp liver under DFB stress.
In this study, carp (Cyprinus carpio) was selected as the research object, and exposure experiments for 15 d under 0.1 mg/L and 1.0 mg/L medicated bath concentrations were carried out with three parallel for each concentration. High-throughput sequencing of the liver was performed using illumina NovaSeq 6000. Differentially expressed genes (DEGs) were screened by P-adjust < 0.05 and |log2FC| ≥ 1, and bioinformatics analysis such as GO functional annotation and KEGG enrichment analysis were performed.The transcriptome analysis results showed that 2 406 DEGs and 2 688 DEGs changed significantly at 0.1 mg/L and 1.0 mg/L exposure concentrations, respectively, and 821 DEGs were co-expressed between the two groups. GO analysis results showed that DEGs in DFB exposure group were enriched in biological processes, cell composition and molecular functions. KEGG enrichment analysis showed that DEGs in low concentration of DFB exposure group were significantly enriched in metabolic pathways such as biodegradation and metabolism of xenobiotics, lipid metabolism, carbohydrate metabolism, amino acid metabolism, signal molecules and interactions, endocrine system and immune system. In addition to the above metabolic pathways, DEGs in high concentration of DFB exposure group were also significantly enriched in metabolic pathways such as folding, classification and degradation, transport and catabolism.
Studies have shown This study showed that DFB exposure caused the disorder of biodegradation and metabolism of xenobiotics, lipid metabolism, carbohydrate metabolism and amino acid metabolism, and generated endoplasmic reticulum stress, inflammatory response and immune toxicity. In summary, the results of this study provide basic data and theoretical basis for further study on the molecular mechanism of DFB stress in carp. |
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