文章摘要
光质对木下紫菜丝状体生长发育的影响
The impact of light quality on the growth and development of Pyropia kinositae conchocelis
投稿时间:2024-04-24  修订日期:2024-05-23
DOI:
中文关键词: 木下紫菜  光质  苗种培育  丝状体  壳孢子
英文关键词: Pyropia kinositae  Light quality  Seedling cultivation  Conchocelis  Conchospores
基金项目:
作者单位邮编
段昱琪 浙江海洋大学水产学院 舟山 316000
梁洲瑞 海水养殖生物育种与可持续产出全国重点实验室 中国水产科学研究院黄海水产研究所 青岛 
徐慧 海水养殖生物育种与可持续产出全国重点实验室 中国水产科学研究院黄海水产研究所 青岛 
鲁晓萍 海水养殖生物育种与可持续产出全国重点实验室 中国水产科学研究院黄海水产研究所 青岛 
袁艳敏 海水养殖生物育种与可持续产出全国重点实验室 中国水产科学研究院黄海水产研究所 青岛 
汪文俊* 海水养殖生物育种与可持续产出全国重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071
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中文摘要:
      木下紫菜(Pyropia kinositae)是理想的紫菜离岸栽培新种类,其苗种培育技术尚待完善。光是藻类生长发育的重要能量源和调控信号,而光质作为光环境的关键组成要素调控紫菜生长发育进程的研究仍鲜见报道。本实验探究了不同光质(红光、绿光、蓝光和白光)对自由丝状体的营养扩增,贝壳丝状体营养藻丝向孢子囊枝的转化促熟,孢子囊枝细胞形成双分的诱导,以及壳孢子放散和萌发等种苗生产关键过程的影响。结果显示:蓝光组自由丝状体的相对生长速率(RGR)显著大于其它光质组,而红光、绿光和白光三组的相对生长速率之间无显著差异,表明蓝光较适宜木下紫菜自由丝状体的营养扩增;蓝光下,自由丝状体的光系统Ⅱ实际光合效率[Y(Ⅱ)]、潜在最大相对电子传递速率(rETRmax)和半饱和光强(Ek),以及贝壳丝状体的光系统Ⅱ最大光合效率(Fv/Fm)均显著大于其它光质组,表明蓝光显著提升了丝状体的光能转换效率,且提高了自由丝状体的电子传递活性和耐受强光能力;与蓝光组相反,红光下自由丝状体的Y(Ⅱ)和rETRmax均显著低于其它光质组。蓝光下,贝壳丝状体营养藻丝向孢子囊枝的转变率(48.7%)及双分孢子形成率(26.7%)均显著高于其它光质组,红光和绿光下的孢子囊枝比例最小(23.1%和24.6%),显著低于蓝光和白光(33.2%);且红光下的孢子囊枝形成双分孢子比例最小(10.9%),显著低于其他光质组。表明蓝光对贝壳丝状体的同步促熟效果显著,白光和绿光的促熟效果次之,红光则较差。对孢子囊枝成熟度一致(双分孢子比例水平相当)的贝壳丝状体进行降温诱导壳孢子放散,早期壳孢子放散量在白光、红光和绿光下无显著差异,而在蓝光下显著增大,表明蓝光可促进双分孢子的释放。红光和绿光下的壳孢子萌发率较低(<10%),而蓝光下的壳孢子萌发率(>80%)显著高于其他光质组。本研究初步探明了木下紫菜丝状体促长促熟及壳孢子放散和萌发的光质条件及其光合生理基础,可为其种苗生产的光环境调控提供理论支撑。
英文摘要:
      Pyropia kinositae is an ideal candidate for offshore cultivation of laver, but there is room for improvement in seedling breeding techniques. Light serves as a crucial energy source and regulatory signal for algal growth and development. However, research on the role of light quality in regulating the growth and development of laver is still scarce. This study extensively investigated the effects of different light qualities (red, green, blue, and white light) on the vegetative conchocelis, the transition from vegetative conchocelis to conchosporangial phase, formation of bipartite cells in conchosporangia, and conchospore release of P. kinositae. We also examined the chlorophyll fluorescence characteristics of conchocelis under different light qualities. The results showed that the relative growth rate of conchocelis under blue light was significantly higher than that under other light qualities, indicating that blue light is more suitable for the expansion of vegetative conchocelis. Under blue light, the actual quantum yield [Y(Ⅱ)], the maximum electron transport rate (rETRmax), and the minimum saturating irradiance (Ek) of conchocelis, as well as the maximal quantum yield of Photosystem II (Fv/Fm) of conchospores, were significantly higher than those under other light qualities. This suggests that blue light significantly enhances the photosynthetic efficiency of conchocelis, and improves the electron transfer activity and phototolerance of conchocelis under high light conditions. In contrast, Y(Ⅱ) and rETRmax of conchocelis under red light were significantly lower than those under other light qualities. Under blue light, the conversion rate of conchocelis to conchospores(48.7%) and the rate of bipartite cells in conchosporangia formation(26.7%) were significantly higher than those under other light qualities, indicating that blue light significantly promotes the synchronous maturation of conchocelis, followed by white and green light, while red light has a poorer effect. When inducing the conchospore release from conchocelis with consistent maturity (similar rates of bipartite cells in conchosporangia formation), there was no significant difference in the early release of conchospores under white, red, and green light, but it significantly increased under blue light, indicating that blue light promotes the release of bipartite cells. The germination rate of conchospores under red and green light was low (<10%), while under blue light, it was significantly higher (>80%) than that under other light qualities. These results provide preliminary insights into the light quality conditions and photosynthetic physiological basis for promoting the growth, maturation, and dispersal of P. kinositae conchocelis and conchospores, offering theoretical support for the light environment regulation in seedling production.
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