文章摘要
温度对红毛菜叶绿素荧光参数及抗氧化系统的影响
Effects of temperature on Chlorophyll Fluorescence Parameters and Antioxidant System of Bangia fuscopurpurea
投稿时间:2024-04-25  修订日期:2024-05-27
DOI:
中文关键词: 温度  红毛菜  叶绿素荧光  抗氧化系统
英文关键词: Temperature  Bangia fuscopurpurea  Chlorophyll fluorescence  Antioxidant system
基金项目:
作者单位邮编
林晓希 浙江海洋大学 水产学院 浙江 舟山 266071
汪文俊* 海水养殖生物育种与可持续产出全国重点实验室 中国水产科学研究院黄海水产研究所 山东 青岛 266000
袁艳敏 海水养殖生物育种与可持续产出全国重点实验室 中国水产科学研究院黄海水产研究所 山东 青岛 
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中文摘要:
      红毛菜(Bangia fuscopurpurea)具有很高的营养价值,是我国特色栽培海藻种类。在当前气候变化背景下,研究红毛菜对不同温度尤其对高温的响应,对指导耐高温品种的选育、促进产业的可持续健康发展具有重要意义。本文通过研究不同温度(5、10、15、20和25℃)下,红毛菜的PSII最大荧光产量(Fv/Fm)、最大相对电子传递速率(rETRmax)、丙二醛(MDA)含量、可溶性蛋白(SP)含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、抗坏血酸过氧化物酶(APX)活性和谷胱甘肽还原酶(GR)活性,初步解析了红毛菜在不同温度下的光合和抗氧化生理响应。结果显示:(1)红毛菜Fv/Fm和rETRmax值总体上呈现随温度升高先上升后下降的趋势。在培养周期内,15℃组红毛菜的Fv/Fm和rETRmax均维持在较高的水平;20℃和25℃组在3 d内的Fv/Fm和rETRmax值显著低于其他温度组,但在第5 d时上升到与15-20℃组间相当的水平,显著高于5-10℃组。(2)MDA含量随着温度的升高呈先下降后上升的趋势,15℃组的MDA含量显著低于其他各组;早期时(6 h),MDA含量在低温下(5-10℃)较高,随着处理时间的延长(5 d),MDA含量在高温组(20-25℃)显著上升。(3)SP的含量在15-20℃时最高,高温(25℃)和低温组(5-10℃)间无显著差异。(4)SOD、APX、GR、CAT活性均为在低温和高温时较高,尤其在第5 d时,显著高于15-20℃组。随着培养时间的延长(5 d),GR活性在25℃和10℃组显著上升,CAT活性在5-10℃组显著上升。以上结果表明,15℃较适宜红毛菜的生长,但红毛菜对高温(20-25℃)也具有较强的适应能力,在适应温度胁迫过程,抗氧化酶类发挥了积极作用。
英文摘要:
      Bangia fuscopurpurea, with its high nutritional value, is a characteristic cultivated seaweed species in China. Against the backdrop of current climate change, investigating the response of B. fuscopurpurea to different temperatures, particularly high temperatures, is significant for guiding the breeding of heat-tolerant varieties and promoting the sustainable and healthy development of the industry. This study elucidates the photosynthetic and antioxidative physiological responses of B. fuscopurpurea to different temperatures (5, 10, 15, 20, and 25°C) by examining parameters such as the maximum fluorescence yield of photosystem II (Fv/Fm), maximum relative electron transport rate (rETRmax), malondialdehyde (MDA) content, soluble protein (SP) content, superoxide dismutase (SOD) activity, catalase (CAT) activity, ascorbate peroxidase (APX) activity, and glutathione reductase (GR) activity. The results reveal that: (1) Overall, Fv/Fm and rETRmax of B. fuscopurpurea exhibit a trend of initially increasing and then decreasing with temperature elevation. Throughout the cultivation period, B. fuscopurpurea maintained relatively high levels of Fv/Fm and rETRmax at 15°C; however, within the first 3 days, Fv/Fm and rETRmax values at 20°C and 25°C were significantly lower compared to other temperature groups, but by day 5, they rose to levels comparable to those of the 15-20°C group, significantly higher than those of the 5-10°C group. (2) The MDA content initially decreased and then increased with temperature elevation, with the MDA content at 15°C significantly lower than that of other groups. Early on (6h), MDA content was higher at low temperatures (5-10°C), but with prolonged treatment time (5 days), MDA content significantly increased in the high-temperature groups (20-25°C). (3) The SP content was highest at 15-20°C, with no significant difference between the high temperature (25°C) and low temperature (5-10°C) groups. (4) SOD, APX, GR, and CAT activities were generally higher at low and high temperatures, particularly on day 5, significantly higher than those of the 15-20°C group. With prolonged cultivation time (5 days), GR activity significantly increased in the 25°C and 10°C groups, while CAT activity significantly increased in the 5-10°C group. These results suggest that 15°C is more suitable for the growth of B. fuscopurpurea, but it also exhibits strong adaptability to high temperatures (20-25°C). During temperature stress acclimationadaptation, antioxidative enzymes play a positive role.
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