| 王妍妍,柳学周,徐永江,刘新富.条斑星鲽染色体带型研究.渔业科学进展,2011,32(4):6-13 |
| 条斑星鲽染色体带型研究 |
| Study on chromosome banding patterns of Verasper moseri |
| 投稿时间：2010-07-23 修订日期：2011-02-23 |
| DOI： |
| 中文关键词: 条斑星鲽 C-带 Ag-NORs带 G-带 |
| 英文关键词: Verasper moseri C-binding Ag-NORs G-banding |
| 基金项目:国家鲆鲽类产业技术体系建设项目（CARS-50）、国家“863”计划项目（2006AA10A414；2010AA10A402）和公益性农业行业专项项目（nyhyzx07-046）共同资助 |
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| 中文摘要: |
| 开展了条斑星鲽染色体C-带、Ag-NORs带及G-带的研究。结果发现，条斑星鲽染色体显示较为复杂的C-带，有4对染色体整体呈阳性深染，分别为3、13、20、22号染色体；3对具居间C-带，分别为1、4、8号染色体；9号染色体整体显示阴性，其余有大小不一的着丝粒C-带。计算其异染色质含量为54.91%。银染分裂相中Ag-NORs出现的数目以两个的频率最高，达87.5%，与此相一致，在间期核中通过银染表现出两个核仁的间期核数目的频率也最高,故确定条斑星鲽染色体具有1对Ag-NORs，位于亚中部着丝点染色体的短臂上，为端部Ag-NOR。Ag-NORs的数目为1～3个，以出现两个Ag-NOR的频率最高，达87.5%，与此相同的是，在间期核中，通过银染表现出两个核仁的间期核数目的频率也最高。G-带研究显示，条斑星鲽有37条深染带，22条浅染带。 |
| 英文摘要: |
| The C-binding, Ag-NORs, and G-banding patterns of the metaphase chromosomes of barfin flounder Verasper moseri were investigated in this study. The results showed that the interstitial regions of most chromosomes exhibited positive C-bands, in which the whole 3rd, 13th, 20th, and 22nd pair of chromosomes showed totally positive C-band, whilst the 1st, 4th and 8th pair of chromosomes only showed positive C-bands in the middle region, the rest with centromeric positive C-bands. However, the 9th pair of chromosomes showed negative result. The C-banding heretochromatin occupied 54.91% of the total chromosome in the present study. Silver staining study showed that most chromosomes featured two silver-stained nuclear organizing regions (Ag-NORs) with the percentage of 87.5%. Accordingly, the interphase nucleus with 2 nucleolus appeared most frequently, thus it is supposed that the barfin flounder bears a pair of Ag-NORs which located at the end of short arm of the submetacentric chromosomes. A total of 59 positive G-bands (37 strong, 22 light) were recorded in 23 pairs of chromosomes. These results would provide basic data for the genomic research and biodiversity conservation of barfin flounder. |
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