温度和金属离子对干扰素诱导剂 聚肌胞配对的影响

毛明光; 王印庚; 尹立杰; 张家松; 刘宗柱

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温度和金属离子对干扰素诱导剂聚肌胞配对的影响
毛明光¹, 王印庚², 尹立杰³, 张家松², 刘宗柱⁴
1.（1青岛农业大学生命科学学院，266109）（2中国水产科学研究院黄海水产研究所，青岛 266071）;2.（2中国水产科学研究院黄海水产研究所，青岛 266071）;3.（3青岛汉河动植物药业有限公司，266103）;4.（1青岛农业大学生命科学学院，266109）

摘要:

为提高聚肌胞（Polyinosinic polycytidylic acid, polyI:C）的配对效果，本文研究了温度和金属离子对聚肌胞配对的影响。实验结果显示，在45℃条件下合成的聚肌胞减色效应最强，说明在该温度下聚肌苷酸（Polyinosinic acid, PI）和聚胞苷酸（Polycytidylic acid, PC）配对最紧密；Na+、Ca2+、Mg2+、Mn2+和Zn2+能够增强聚肌胞双链结构的紧密性，当Na+浓度增加到0085mol/L后，聚肌胞的配对接近饱和； Ca2+、Mg2+、Mn2+和Zn2+对聚肌胞结构的保护效果基本一致，而Cu2+对聚肌胞结构具有明显破坏作用。建议采用45℃合成聚肌胞并添加适当浓度的Na+和Ca2+等增强其结构的紧密性，以避免机体内高活性RNA酶的降解而失去药效。

关键词: 干扰素诱导剂聚肌胞配对温度金属离子

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基金项目:国家863计划项目（2003AA622070）和国家科技部十一五科技支撑计划项目与养殖技术示范（2006BAD09A11）共同资助

Effects of temperature and metallic cations on the combination of interferon inducer polyinosinic polycytidylic acid (polyI:C)

Abstract:

Effects of temperature and metallic cations on the synthesis of polyinosinic polycytidylic acid were tested to improve its structure. The results showed significant hypochromic effects of polyI:C formed at 45℃，which indicated that the best combination of polyI:C were achieved at this temperature, while the metallic cations including Na+、Ca2+、Mg2+、Mn2+ and Zn2+ could enhance the polyI:C combination. 0085mol/L PolyI:C combination achieved saturation when Na+ concentration was, and the divalent cations including Ca2+、Mg2+、Mn2+ and Zn2+ played positive effect on the combination. However, Cu2+ tend to destroy the double stranded structure. It is suggested that polyI:C should be made at 45℃ and suitable concentration of Na+ and Ca2+should be added to make its structure more tight in order to avoid RNase’s digesting. This result is useful for synthesizing polyI:C at a high quality.

Key words: Interferon inducer Polyinosinic-polycytidylic acid Synthesis Temperature Metallic cations