| 摘要: |
| 通过研究冻融海蜇触手，Sephadex G 10凝胶过滤层析脱盐，SP Sephadex C 25离子交换层析等分离纯化技术，从海蜇Rhopilema esculentum触手中分离到RE 5 6毒素，初步测定其分子量约为662 kD并对其性质进行了初步研究。结果表明，RE 5 6具有下列性质。（1）对鸡血表现出较强的溶血活性；（2）可大大提高小白鼠的对热痛阈值即有明显的镇痛效果，且镇痛作用随着注射浓度的增加而加强；（3）对小白鼠皮肤和眼睛分别表现中度和轻度刺激性；（4）对革蓝氏阴性菌大肠杆菌以及真菌白色念珠菌等有抑制作用。但未检测到丝氨酸蛋白酶抑制剂活性。 |
| 关键词: 海蜇 毒素 溶血活性 刺激实验 |
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| 基金项目:江苏省高校自然科学基金项目（06KJD240012）、江苏省海洋生物技术重点建设实验室开放课题基金项目(2006HS015)和连云港市2005年度支柱产业科技攻关项目(CN0540)共同资助 |
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| Preliminary study on isolation, purification and characteristics of a toxin from jellyfish |
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| Abstract: |
| A toxin, named RE 5 6，was isolated, purified and characterized from the tentacles of Rhopilema esculentum by Sephadex G 10 gel filtration and SP Sephadex C 25. The molecular weight was 662 kD. RE 5 6 showed hemolytic activity to chicken blood; its analgesic effect to mice was significant and intensified with concentration increasing; it showed medium or low level stimulation the skin and eyes of mice; it also inhibited the growth of Escherichia coli and Candida albicans. No serine protease inhibiting activity was revealed by the examination.
KEY WORDSJellyfish Rhopilema esculentumToxinHaemolytic activity |
| Key words: Jellyfish Rhopilema esculentumToxinHaemolytic activity
Stimulative test |
