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浅海养殖环境复合生态净化菌群的筛选及其净化功能
李洪鹏1, 李秋芬2, 张艳2, 李筠3
1.(1中国海洋大学海洋生命学院,青岛 266003 ) (2农业部海洋渔业资源可持续利用重点开放实验室 中国水产科学研究院黄海水产研究所,青岛 266071);2农业部海洋渔业资源可持续利用重点开放实验室 中国水产科学研究院黄海水产研究所,青岛 266071;3.1中国海洋大学海洋生命学院,青岛 266003
摘要:
本研究由浅海网箱区富营养沉积物经多步富集和筛选获得高效复合生态净化菌群,对浅海养殖区的有机物、氨氮和亚硝酸氮有明显去除效果。研究了不同条件对复合菌液去除养殖水体中氨氮、亚硝酸氮和有机物能力的影响,并确定了最佳净化条件。结果表明,复合菌添加量、葡萄糖添加量,处理时间、温度、pH和盐度对复合菌的去除效果均有影响,实验条件确定为复合菌的添加量为3%、处理时间为4 d、温度为30±2 ℃、pH值为8.1±0.2、葡萄糖添加量为2 g/L和盐度为(30±10) g/L 时,去除效果达到最佳,此时氨氮、亚硝酸氮和溶解有机物的去除率可分别达到79.1%、85.2%和88.7%。
关键词:  养殖环境  复合菌  生物修复  异养硝化菌
DOI:
分类号:
基金项目:国家863探索项目(2006AA10Z414、2006AA10Z415)、海洋公益性行业科研专项经费项目(200805069)和山东省博士后科研资助项目(200601011)共同资助
Screening of eco-compound bacteria from coastal aquaculture environment and study on their purification ability
Abstract:
In this study, a group of compound heterotrophic and nitrifying bacteria was enriched and screened from inshore aquaculture environment. And several influencial factors for their purification ability were investigated. The experiment results showed that, the amount of added bacteria, glucose, temperature, pH and salinity all influenced their pollutant removing rate. When the amount of added combination of bacteria was 3%,time of treatment was 4 days, at temperatures 30±2 ℃, pH 8.1±0.2,salinity of 30±10 g/L and 2 g/L glucose was added, the purification effect was the best, resulting in a removal rate of 79.1% for ammonia, 85.2% for nitrite and 88.7% for COD, respectively. This study provided a theoretical basis for bioremediation of inshore aquaculture environment by compound bacteria.
Key words:  Aquaculture environment  Eco-compound bacteria  Bioremediation  Heterotrophic nitrifying bacteria