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悬浮物对半滑舌鳎胚胎和初孵仔鱼的毒性效应
周勇1, 马绍赛2, 曲克明2, 陈聚法2, 徐勇2
1.1上海海洋大学水产与生命学院, 201306) (2农业部海洋渔业资源可持续利用重点开放实验室 中国水产科学研究院黄海水产研究所,青岛 266071);2农业部海洋渔业资源可持续利用重点开放实验室 中国水产科学研究院黄海水产研究所,青岛 266071
摘要:
研究了不同浓度悬浮物对半滑舌鳎Cynoglossus semilaevis Günther胚胎和初孵仔鱼的毒性效应。实验结果表明,悬浮物对半滑舌鳎胚胎的孵化时间影响不明显,悬浮物浓度 ≤ 200 mg/L 时,胚胎孵化率与对照组无明显差异(P>005),悬浮物浓度≥ 800 mg/L时,其胚胎孵化率与对照组有极显著差异(P<0.01),悬浮物浓度≥ 200 mg/L 时,初孵仔鱼畸形率与对照组有极显著差异(P<0.01)。通过Bliss法计算,悬浮物对半滑舌鳎胚胎的起始半致死浓度(95%可信限)为 1 974.8 mg/L (1 375.4-2 835.5 mg/L),安全浓度(SC)为 197.5 mg/L;对初孵仔鱼的 48和 96 h LC50 (95%可信限) 分别为 226.9 mg/L (204.6-251.7 mg/L) 和 202.9 mg/L (182.3-225.9 mg/L),SC为 20.29 mg/L。
关键词:  悬浮物  半滑舌鳎  胚胎  初孵仔鱼  毒性效应
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基金项目:国家科研院所科研专项资金项目(2060302/2)资助
Toxic effects of suspended substances (SS) on the embryos and newly hatched larvae of Cynoglossus semilaevis Günther
Abstract:
Studies on the toxic effects of suspended substances (SS) on the embryos and newly hatched larvae of Cynoglossus semilaevis  Günther were conducted in laboratory in October 2007. The results showed that the effect of SS on hatching time was not evident. When the SS concentration was less than 200 mg/L, there was no significantly difference in hatching rate (P>0.05)between the treatment and control.When the SS concentration was more than 200 mg/L, there were significant difference between the treatment and control in embryos hatching rate and deformity rate of newly hatched larvae (P<0.01). The Incipient LC50(95% confidence limits)of SS to embryo was 1 974.8 mg/L (1 375.4-2 835.5 mg/L), and the safety concentration(SC) was 197.5 mg/L.The 48 h LC50 and 96 h LC50(95% confidence limits)of SS to newly hatched larvae were 226.9 mg/L (204.6-251.7 mg/L) and 202.9 mg/L (182.3-225.9 mg/L), respectively,and the SC was 20.29 mg/L.
Key words:  Suspended substances (SS)  Cynoglossus semilaevis Günther  Embryos  Newly hatched larvae  Toxic effects