| 摘要: |
| 在封闭循环水养殖条件下，进行5×3双因素随机设计动物试验，即5种饲料蛋白质水平（43%、46%、49%、52%和56%；以A—E组表示）和3种饲喂饱食度水平（100%、90%和80%；以Ⅰ、Ⅱ和Ⅲ水平表示），共15个处理，每处理3重复，试验期108 d。探寻蛋白质营养与饱食度对工厂化养殖半滑舌鳎幼鱼（110±25 g）生长与免疫的影响。结果表明,（1）高蛋白水平与高饱食度投喂的增重效果最佳，E组增重率极显著高于其他组13.75%-50.16%,Ⅰ水平比Ⅱ和Ⅲ水平分别极显著提高7.57%和14.08%；中蛋白水平蛋白效率较高而死亡率最低，C组死亡率比其他组降低50%-75%；低饱食度的饲料利用率和蛋白质效率最高，Ⅲ水平比Ⅰ、Ⅱ水平分别显著高4.78%和5.32%。（2）中蛋白水平和高饱食度有利于提高超氧化物歧化酶（SOD）活力，C组比其他组显著提高4.2%-34.79%，Ⅰ水平分别比Ⅱ和Ⅲ水平极显著高15.27%和25.0%；中蛋白水平和90%饱食度有利于提高溶菌酶（LZM）活力，C组比其他组极显著提高4.61%-18.07%，Ⅱ水平比Ⅰ和Ⅲ水平分别极显著高12.03%和4.58%；中高蛋白水平和高饱食度有利于提高补体C3和C4活力。（3）获得工厂化养殖半滑舌鳎幼鱼最大生长的饲料蛋白质水平为56%；最佳免疫力和蛋白效率的饲料蛋白质水平为49%～52%。 |
| 关键词: 半滑舌鳎 蛋白水平 饱食度 生长性能 免疫力 |
| DOI： |
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| 基金项目:国家高技术研究发展计划重点项目(2006AA100305)资助 |
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| Effects of protein and satiation degrees on growth and immunity of Cynoglossus semilaevis Günther in industrial culture |
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| Abstract: |
| he 5×3 two factor random animal experiment was conducted for 108 days to determine the effects of protein levels and satiation degrees on growth performance and immunity activity of juvenile Cynoglossus semilaevis Günther (with average initial weight of 110±25g) in the industrial culture condition with closed recirculation seawater. The trial fish were randomly allocated into 15 triplicated treatments,with five diets at protein levels of 43%，46%，49%，52%，56%(A E groups）and three satiation degrees of feeding（100%，90% and 80%, asⅠ和Ⅱ and Ⅲdegree）. The results indicated that: (1)the best weight gain were found in both the highest protein level and the highest satiation degree. Weight gain ratio in group E was significantly higher than the other groups (13.75%-50.16%),and that of degree Ⅰ was significant higher than degree Ⅱand Ⅲ (7.57% and 14.08%), respectively; higher protein efficiency ratio and the minimum mortality appeared in the intermediate protein level , mortality ratio of group C was lower than the others (50%-75%) the highest feed conversion ratio and protein efficiency ratio were present in the lowest satiation degree,for which degree Ⅲ was higher than the others (4.78% and 5.32%), respectively .(2) the intermediate protein level and the highest satiation degree were stimulative to enhancing SOD activity, group C was significantly higher than the other groups (4.2%-34.79%) degreeⅠwas significant higher than degree Ⅱ and Ⅲ (15.27% and 25.70%),respectively; the intermediate protein level and 90% satiation were good for promoting LZM activity, group C was significant higher than the other groups (4.61%-18.07%), degree Ⅱ was significant higher than degree Ⅰ and Ⅲ (12.03% and 4.58%), respectively; the intermediate and higher protein level and the highest satiation degree were stimulative to enhancing complement C3，C4 activity; (3) the protein level of feed for maximum growth of juvenile C. semilaevis Günther in the industrial culture condition was 56%, but for optimal immunity activity and protein efficiency ratio was 49%-52%. |
| Key words: Cynoglossus semilaevis Günther Protein levels Satiation degree Growth performance Immunity activity |
