| 摘要: |
| 以PolyI∶C（又称聚肌胞）为诱导剂，分别通过腹腔注射、浸泡和投喂3种途径诱导大菱鲆 Scophthalmus maximus体内抗病毒蛋白Mx基因的转录，利用半定量RT-PCR方法检测干扰素下游基因-Mx基因的转录水平来确定该诱导剂的诱导效果。结果显示，以上3种途径都能高效诱导Mx蛋白mRNA的转录，均在48h之后达到高峰，其中以浸泡的方式更容易诱导Mx基因转录，且在120h时仍保持较高水平。Mx基因转录的时相变化证明了国产PolyI∶C可以通过多种途径诱导抗病毒蛋白Mx的表达，为实际应用中确定用药途径提供了理论依据。另外，实验初步建立了半定量RT-PCR方法，为检测鱼体内干扰素的表达提供了技术方法。 |
| 关键词: PolyI∶C 大菱鲆 Mx蛋白 干扰素诱导剂 基因转录 |
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| 基金项目:国家863计划（2003AA622070）、国家‵十一五′科技支撑计划（2006BAD09A11）和行业专项（nyhyzx07-046）共同资助 |
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| Transcription of turbot Scophthalmus maximus Mx gene induced by polyinosinic polycytidylic acid given in different ways |
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| Abstract: |
| Transcription of turbot Mx protein confirmed to be an antivirus factor was induced by PolyI∶C with intraperitoneal injection, immersion and oral administration. Mx mRNA was tested by semi-quantitative RT-PCR to validate the effect of PolyI∶C’s induction. The results illustrated that all the three induction ways could effectively induce the transcription of Mx gene and the peak of Mx mRNA quantity appeared at 48h after PolyI∶C application. The immersion method seemed to be the most effective way to induce Mx mRNA, as Mx mRNA could last more than 120 h at a high level. This experiment proved that the homemade PolyI∶C could effectively induce Mx mRNA transcription in different applications, which provided a reference to use PolyI∶C in aquaculture practice. |
| Key words: Polyinosinic-polycytidylic acid (PolyI∶C) Scophthalmus maximus Mx protein Interferon inducer Gene transcription |
