| 摘要: |
| 采用琼脂扩散法(打孔法)测定了五倍子等18种中草药对鳗弧菌、副溶血弧菌、河口弧菌及霍乱弧菌等4种病原弧菌的抑制作用。结果表明，五倍子、苏木、地锦草、石榴皮、黄芩、虎杖和山楂等7种中草药对病原弧菌具有较好的抑菌效果，而金银花等11种中草药抑菌作用较弱；同时采用试管连续稀释法，测定了五倍子等抑菌效果较强的7种中草药对4种病原弧菌的抑菌和杀菌浓度。结果表明，苏木、五倍子、地锦草和石榴皮4种中药具有较好的抑菌和杀菌作用，此4种中药对鳗弧菌、副溶血弧菌、河口弧菌及霍乱弧菌等4种病原弧菌的最小抑菌浓度（MIC）为0.78~6.25 mg/ml，最小杀菌浓度（MBC）为3.12~12.5 mg/ml。 |
| 关键词: 中草药 弧菌 最小抑菌浓度 最小杀菌浓度 |
| DOI： |
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| 基金项目:江苏省自然科学基金项目（BK2009163）、“十一五”国家科技支撑计划重大项目(2006BAD09A01)和连云港市科技攻关项目（CN0826）共同资助 |
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| Study on the antibacterial effects of common Chinese herbs against four pathogenic Vibrio sp. in vitro |
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| Abstract: |
| The inhibitory effects of 18 Chinese herbs on Vibrio anguillarium, V. prarhaemalyticus, V. aestuarianus and V. cholerae were tested by disc diffusion method (punch method). The results showed that 7 Chinese herbs including Galla chinensis, Caesalpinia sappan, Herba euphorbiae, pomegranate bark, Radix scutellariae, Polygonum cuspidatum and Fructus Crataegi can effectively inhibit the growth of 4 pathogenic Vibrio sp., and the other 11 Chinese herbs showed less inhibition. At the same time, the minimal inhibitory concentration and the minimum bactericidal concentration of the 7 Chinese herbs which had significant inhibitory effects to Vibrio sp. were tested by the tube dilution.The results showed that Caesalpinia sappan, Galla chinensis, Herba euphorbiae and Pomegranate bark had significant inhibitory and bactericidal effects, and the minimal inhibitory concentrations of Caesalpinia sappan, Galla chinensis, Herba euphorbiae and Pomegranate bark for 4 pathogenic Vibrio sp. were among 0.78~6.25 mg/ml, the minimal bactericidal concentrations were 3.12~12.5 mg/ml. |
| Key words: Chinese herbs Vibrio sp. Minimal inhibitory concentration Minimum bactericidal concentration |
