| 摘要: |
| 在室内模拟条件下，研究了不同暴露浓度和不同作用时间下胜利原油对半滑舌鳎幼鱼肝脏超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性的影响，以探讨其用于石油污染的生物标志物的可行性。结果表明，从时间效应关系分析，半滑舌鳎幼鱼肝脏SOD和CAT活性随时间总体呈现升高降低.升高的趋势，并且随着暴露浓度的增加，出现高值的时间提前。从剂量.效应关系分析，半滑舌鳎幼鱼肝脏SOD和CAT活性总体呈现低浓度诱导，高浓度抑制的过程。污染物对生物体的毒性效应存在一定的阈值，在这个阈值内机体未发生中毒反应，污染胁迫解除后也能较快地恢复到正常水平。在本实验中，石油污染对半滑舌鳎幼鱼肝脏CAT活性影响的阈值在0.2~0.4 mg/L之间，而对SOD活性影响的阈值在1.6~3.2 mg/L之间。在此阈值以上，表明石油污染导致机体产生活性氧的速度超过机体抗氧化防御系统的清除能力，损伤作用占主导地位，使机体发生毒性效应。半滑舌鳎幼鱼肝脏CAT活性对石油污染的响应相对于SOD更加敏感，所以更能反映出环境中氧化污染的胁迫，有望成为石油类污染的生物标志物。 |
| 关键词: 胜利原油 半滑舌鳎 超氧化物歧化酶 过氧化氢酶 生物标志物 |
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| 基金项目:东省自然科学青年基金（ZR2010CQ023）、国家863计划项目（2007AA10Z239）、国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放基金（200917）和中国水产科学研究院黄海水产研究所基本科研业务费专项基金（2010-ts-01）共同资助 |
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| Effect of Shengli crude oil on the activities of superoxide dismutase and catalase in the liver of Tongue sole Cynoglossus semilaevis |
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| Abstract: |
| Under indoor simulation conditions, the effects of various concentrations and exposure time of Shengli crude oil on the activities of superoxide dismutase (SOD) and catalase (CAT) in the liver of juvenile Cynoglossus semilaevis were studied. The change of SOD and CAT activities versus time showed an increase-decrease-increase trend, and the time for SOD activities to reach the peak value was shorter when exposed to the higher concentrations. In terms of the dose-effect relationship, the activities of SOD and CAT were induced by oil treatment at the lower concentrations and were suppressed at the higher concentrations. A threshold of toxic effects of the oil on the organism was observed, below which there was no toxicity, and the activities of SOD and CAT recovered to the normal level quickly after being released from the pollution stress. In this study, the threshold for CAT was in the range of 0.2~0.4 mg/L, and 1.6~3.2 mg/L for SOD. Beyond the threshold value, the rate of active oxygen production induced by the petroleum pollutant overtook the anti-oxidation eliminating activity of the defense system of the organism, incurring damage to the poisoned organism. The response of CAT activity to the petroleum pollutant was more sensitive than SOD, indicating that CAT can better reflect pollution stress and can be a bio-indicator candidate for petroleum pollution. |
| Key words: Shengli crude oil Cynoglossus semilaevis Superoxide dismutase Catalase |
