| 摘要: |
| 分析了克氏原螯虾Procambarus clarkia人工感染白斑综合征病毒(White Spot Syndrome Virus,WSSV)后，其血细胞吞噬(Phagocytosis)活性，肝胰腺酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活性的变化规律，其目的是为WSSV感染与螯虾的免疫防御反应等研究提供依据。分析结果显示，随着WSSV感染克氏原螯虾时间的延长，血细胞吞噬活性、肝胰腺酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性也随之改变，其中血细胞吞噬活性的变化规律性比较明显，可作为WSSV感染的敏感指标，也可以用作间接指示克氏原螯虾健康状况的指标。而在不同的感染时间，克氏原螯虾肝胰腺ACP与AKP的活性波动较大，无明显的规律可循，难以作为WSSV感染的敏感指标。 |
| 关键词: WSSV 克氏原螯虾 吞噬活性 碱性磷酸酶 酸性磷酸酶 |
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| 基金项目:国家重点基础研究发展规划(973)项目(2006CB101806)和公益性行业(农业)科研专项(200803012)共同资助 |
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| Impacts of WSSV on the activity of haemocyte phagocytosis and hepatopancreas phosphatase in Procambarus clarkia |
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| Abstract: |
| The impacts of White Spot Syndrome Virus (WSSV) on the haemocyte phagocytic activity,and the acid phosphate (ACP) and alkaline phosphate (AKP) activities in the hepatopancreas of Procambarus clarkia were examined.The results showed that the immune factors of P.clarkia changed along with the WSSV infection time,and phagocytes activity could reflect the health of shrimps indirectly.However,the variation of the ACP and AKP activity was not obvious and had no apparent tendency during different infection stages.This study provided basic data on immune level change after pathogenic affection. |
| Key words: WSSV Procambarus clarki Phagocytosis AKP ACP |
