| 摘要: |
| 应用扫描电镜和透射电镜观察了金乌贼精子的超微结构。结果表明，精子全长为191.84μm(n=10)，由头部和尾部组成。其中，头部主要由顶体和细胞核组成，尾部分为中段、主段和末段3个部分。头部顶体为呈倒“U”字形的囊状体，亚顶体腔呈∩形。细胞核细长，呈圆柱状，稍弯曲，为高电子密度的均质结构。核后窝偏离细胞核中心位置。尾部中段较长，由线粒体距及其不完全包围的鞭毛组成。线粒体距分为上段和下段，上段含较多线粒体，下段无线粒体。尾部鞭毛主段细长，由“9+2”结构的轴丝及外围9束粗纤维组成，构成典型的“9+9+2”结构。 |
| 关键词: 金乌贼 精子 超微结构 |
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| 基金项目:国家高科技研究发展计划（863计划）（2010AA10A404）、青岛市科技发展计划（07-1-4-12-hy）和黄海水产研究所基本科研业务费项目（2010-ts-02）共同资助 |
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| Ultrastructure of the spermatozoon of Sepia esculenta Hoyle |
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| Abstract: |
| Ultrastructure of the spermatozoon of Sepia esculenta Hoyle was observed by scanning and transmission electron microscopy. The sperm is 191.84μm (n=10) long and consists of two parts, the head and the tail. The head is mainly composed of acrosome and nucleus, and the tail includes the middle piece, the principal piece, and the end piece. The acrosome was an inverted U-shaped cystid, and the subacrosomal space was ∩-shaped. The nucleus was long, slightly curved and cylindrical-shaped, and was filled with homogeneous texture with high electron density. The posterior nuclear fossa diverged from the center of the nucleus. The middle piece of the tail is very long and consists of mitochondria spur and flagellum incompletely encircled. The mitochondria spur includes two parts, the proximal part and the distal part. The proximal part contained a great amount of mitochondria, while the distal part contained no mitochondria. The principal piece of flagellum is slender, and is composed of a “9+2”axoneme surrounded by nine bundles of coarse fibers, and it formed the typical structure of “9+9+2” |
| Key words: Sepia esculenta Hoyle Golden cuttlefish Spermatozoon Ultrastructure |
