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| 雄性黄颡鱼芳香化酶基因mRNA在性成熟期组织中表达分析 |
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温海深1,2, 刘淼1, 祁保霞3, 何峰1, 李吉方1, 史丹1, 马瑞芹1, 胡建1, 母伟杰1, 张远青1
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1.中国海洋大学水产学院,;2.农业部淡水鱼类遗传育种和养殖生物学重点开放实验室;3.内蒙古民族大学动物科学技术学院
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| 摘要: |
| 在水温分别为20(常温对照组)、25、30℃条件下，采用RT-PCR方法, 研究性腺成熟期雄性黄颡鱼组织中P450芳香化酶(P450aromA和P450aromB)的mRNA表达水平，同时测定性腺成熟系数(GSI)、肝重指数(HSI)、肥满度(CF)和血浆睾酮(T)，雌二醇(E2)含量。结果表明，P450aromA在性腺发育成熟期雄性黄颡鱼心脏、肝、脾、胃、肾脏、精巢、鳃、脑、头肾、肠管组织中均无表达，P450aromB只在脑和肠中有表达，在脑中表达量高于肠，表达量随着水温升高而显著下降；各组实验鱼血浆T和E2含量与芳香化酶基因表达量存在相关关系。 该实验结果表明，P450aromA可能与精子发生相关，可能不参与黄颡鱼精子排放过程；P450aromB在雄鱼脑中高度表达，可能参与性腺成熟期精子活力保持与排放过程，且应激温度越高，对性腺成熟期精子活力保持与排放过程影响越大。 |
| 关键词: 黄颡鱼 P450arom mRNA表达 睾酮 雌二醇 |
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| 基金项目:农业部水生动物遗传育种和养殖生物学重点开放实验室重点课题(BZ200709)资助 |
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| Analysis of P450arom gene mRNA expression inhistology of mature male Pelteobagrus fulvidraco |
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| Abstract: |
| The steady-state level of the P450arom (P450aromA and P450aromB) mRNAs of male Pelteobagrus fulvidraco cultured at different temperatures and growth conditions were analyzed by RT-PCR, and GSI, HSI, CF, serum sexual steroid hormones testosterone (T) and estradiol (E2) levels were measured by 125I radioimmunoassay (RIA). The spatial expression result showed that P450aromA mRNA was not present in ten tissues of male P. fulvidraco, including spermary, liver, stomach, intestines, gill, heart, spleen, kidney, head kidney, and brain. P450aromB was expressed in brain and intestines. Expression of P450aroms was fist linked to temperature, serum sexual steroid hormones testosterone (T) and estradiol (E2) level. The changes in mRNA level of P450arom shared the same trends with serum E2 and T cultured at different temperatures. Our results suggested that P450aromA may be related with spermatogenesis. The high expression level in the brain of the male fish may be directly involved in the maintaining of sperm vitality and spermiation process. The results showed that higher temperature made more significant effects on sperm vitality and spermiation process. |
| Key words: Pelteobagrus fulvidraco P450arom mRNA expression Testosterone Estradiol-17β |