| 摘要: |
| 用含不同浓度梯度（0、50、100、150 mg/kg）黄芩苷的饲料连续投喂健康的中国对虾Fenneropenaeus chinensis，于给药第1、3、5、7、9、11 天采集实验对虾的血淋巴、肝胰腺、肌肉组织，测定不同组织的酸性磷酸酶（ACP）、过氧化氢酶（CAT）、一氧化氮合酶（INOS）、溶菌酶（LSZ）、超氧化物歧化酶(SOD)活性的变化，研究黄芩苷对中国对虾非特异性免疫酶活指标和解毒代谢能力的影响。实验结果表明，中、高浓度处理组血淋巴、肝胰腺、肌肉组织的ACP、INOS、SOD在第3天后呈上升趋势，分别达到峰值后趋于稳定状态，与同期对照组相比差异显著（P<0.05）；低浓度黄芩苷处理组各种酶活性与同期对照组相比差异不显著（P>0.05），而低、中、高浓度处理组血淋巴LSZ酶活性与同期对照组相比差异极显著 (P<0.01)。各种酶活亦存在组织差异性，其中ACP、CAT在肝胰腺中活性最高，肌肉和血淋巴次之；而INOS、LSZ、SOD活性，血淋巴中最高，肌肉和肝胰腺次之。同时攻毒试验表明，黄芩苷能有效地降低试验组中国对虾的死亡率，提高免疫保护率。其中，中浓度处理组的保护效果最佳。 |
| 关键词: 中国对虾 黄芩苷 酸性磷酸酶 过氧化氢酶 一氧化氮合酶 溶菌酶 超氧化物歧化酶 鳗弧菌 |
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| 基金项目:国家高技术研究发展计划（2006AA10A406）、公益性农业行业科研专项（200803012）和科技部农业科技成果转化资金项目（2010GB23260589）共同资助 |
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| Influence of baicalin on immune enzymes and detoxification metabolism of shrimp Fenneropenaeus chinensis |
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| Abstract: |
| The aim of the study was to test the influence of baicalin on immune enzymes and detoxification metabolism of shrimp Fenneropenaeus chinensis. Four treatments were designed with different concentrations of baicalin (0, 50, 100, 150 mg/kg) mixed with the raw diet, and the diets were orally fed to healthy F. chinensis. The activities of acid phosphatase (ACP), catalase (CAT), induced nitric oxide synthase (INOS), lysozyme (LSZ), superoxide dismutase (SOD) in haemolymph, hepatopancreas, and muscle were assayed on days 1, 3, 5, 7, 9 and 11. The data indicated that activities of ACP, INOS, SOD in the 100mg/kg and 150 mg/kg treatments increased significantly (P<0.05) compared to the control level after feeding, and achieved the highest level in three days. All activities in the 50mg/kg treatment were not significant higher than those in the control (P>0.05), while increase of activities of LSZ in heamolymph were highly significant (P<0.01) in the 50, 100 and 150mg/kg treatments compared to the control level at the same period. The activities of (ACP and CAT changed most greatly in hepatopancreas, followed by muscle and hemolymph. The activities of INOS, LSZ, SOD changed most greatly in hemolymph, followed by muscle and hepatopancreas. The middle level treatment and high level treatment reduced significantly (P<0.05) below the control level. Results of the infected test showed that the artificial infection with V. anguillarum caused mortality in all groups, but the mortality of the control group was the highest and the mortality of the medium dose was the lowest. The baicalin elevated the protective rate of F. chinensis infected with V. anguillarum. The results showed that baicalin could regulate the immune system of F. chinensis significantly, enhance non-specific immune function and disease resistance, and depress the susceptivity of F. chinensis effectively. All these results would provide a theoretical basis for the preparation of compound Chinese herbal medicine. |
| Key words: Fenneropenaeus chinensis Baicalin ACP CAT INOS LSZ SOD Vibrio anguillarum |
