| 摘要: |
| 在中国对虾基础饲料中用水解鱼蛋白替代0%、5%、10%和15%鱼粉蛋白，制成饲料投喂中国对虾30d，实验结束取样测定对虾生长指标并进行血细胞记数，以肝胰脏中的酸性磷酸酶、碱性磷酸酶、溶菌酶、超氧化物歧化酶活性和血清中的酸性磷酸酶、碱性磷酸酶、溶菌酶、超氧化物歧化酶、过氧化物酶、一氧化氮合酶活性为非特异免疫指标，探讨水解鱼蛋白作为免疫增强剂对中国对虾非特异性免疫效应的影响。结果表明,5%水解鱼蛋白替代鱼粉蛋白组对虾肝胰脏中酸性磷酸酶活性为0.54 U/g prot，显著高于对照组和其他替代组(P<0.05)；血液中总的血细胞数量最多，血清的酸性磷酸酶、碱性磷酸酶和溶菌酶活性均显著高于对照组和其他替代组(P<0.05)，并且特定生长率最高。说明水解鱼蛋白作为免疫增强剂对中国对虾的非特异性免疫功能具有明显的增强作用。 |
| 关键词: 中国对虾 水解鱼蛋白 非特异性免疫 生长 |
| DOI： |
| 分类号: |
| 基金项目:国际合作专项（2008DFA31720）、公益性（农业）行业专项（200803012）和农业部“948”项目（2010 S11）共同资助 |
|
| Effects of fish protein hydrolysate in the diet on the non-specific immune response of Penaeus chinensis |
|
|
| Abstract: |
| The effect of fish protein hydrolysate(FPH) was examined as an immuno enhancer on the non specific immune response of Penaeus chinensis. P.chinensis were fed with 0%, 5%, 10%, and 15% FPH replacing fish meal protein for 30 days. Samples were collected at the end of the experiment.The results showed that the acid phosphatase activity in the hepatopancreas of P.chinensis fed diet with 5% FPH replacing fish meal protein was significantly higher than that of other groups (P<0.05)，and total haemocyte count of this treatment was significantly higher (P<0.05) than those of other treatments.The activities of acid phosphatase,alkaline phosphatase and lysozyme in the serum of P.chinensis fed with 5% FPH replacing fish meal protein were significantly higher (P<0.05) than those of other groups. Moreover,the group of 5% FPH replacing fish meal protein resulted in a significant SGR increase(P<0.05). In conclusion, fish protein hydrolysate could significantly improve the non-specific immune response of P.chinensis. |
| Key words: Penaeus chinensis Fish protein hydrolysate Non-specific immune Growth |
