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南极磷虾粉对大菱鲆生长、非特异性免疫及氟残留的影响
孔凡华1,2, 梁萌青1, 吴立新2, 郑珂珂1, 王新星1, 常青1, 龚蓉珠3
1.中国水产科学研究院黄海水产研究所 青岛市海水鱼类种子工程与生物技术重点实验室,266071;2.大连海洋大学生命科学与技术学院,116023;3.北京大卫麦克食品香公司,100026
摘要:
以鱼粉、南极磷虾粉和豆粕为蛋白源,以南极磷虾粉蛋白分别替代饲料中的0(对照组)、20%、40%、60%的鱼粉蛋白制成4种等氮等能的饲料,并以此饲料饲喂大菱鲆(37.36±2.12g)56 d。试验结果显示,饲料中南极磷虾粉水平对大菱鲆的成活率及特定生长率(SGR)没有显著性影响(P>0.05);对各组的饲料干物质表观消化率均有显著性影响(P<0.05),替代40%组与替代60%组蛋白质表观消化率较对照组有显著性差异(P<0.05);对大菱鲆血清中的碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、溶菌酶及总超氧化物歧化酶(T-SOD)活力无显著性影响(P>0.05)。大菱鲆各组间肌肉中氟残留量没有显著性差异(P>0.05),且肌肉中氟含量均在可食用范围内,大菱鲆各组间骨骼中氟残留量均有显著性差异(P<0.05)。
关键词:  大菱鲆  南极磷虾粉  生长  非特异性免疫  氟残留
DOI:
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基金项目:公益性行业(农业)科研专项(nyhyzx07-046-鲆鲽)和农业部“南极海洋生物资源开发利用”项目共同资助
Effect of different level of Antarctic krill meal on growth, non-specific immunity and fluoride retention in diets of turbot Scophthalmus maximus
Abstract:
The effects of partial replacement of fish meal (FM) with meal made from Antarctic krill Euphausia superba as protein source in the diets for turbot Scophthalmus maximus on growth, non-specific immunity and fluoride retention were studied. Four experimental diets were prepared using an FM diet as the control. The other diets included Antarctic krill where 20%, 40% or 60% of the dietary FM protein was replaced by the protein from Antarctic krill. All diets were iso-nitrogenous and iso-caloric. Four diets were fed to groups of turbot(37.36±2.12 g) for 8 weeks. Experimental results showed that in fish fed diets containing Antarctic krill, survival, specific growth rate (SGR), alkaline phosphatase(AKP), acid phosphatase (ACP), lysozyme (LZM) and total superoxide dismutase(T-SOD)were significantly different compared with fish fed the control diet(P>0.05). The apparent digestibility of each experimental group has significant difference (P<0.05), and the protein digestibility rate of 3(40) group and 4(60) group had significant difference with the control group (P<0.05). The fluoride retention in muscles of each experimental group has no significant difference (P>0.05), but in bones, each experimental group has significant difference (P<0.05).
Key words:  Turbot  Antarctic krill  Growth  Non-specific immunity  Fluoride retention