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饲料中维生素C对七彩神仙鱼仔幼鱼非特异性免疫的影响
廖畅宇1, 邵力1, 王旭霞1
宁波大学 教育部应用海洋生物技术重点实验室,315211
摘要:
通过投喂维生素C含量为0-2 000.0 mg/kg的饲料,对七彩神仙鱼仔幼鱼非特异性免疫进行了研究。结果表明,当饲料中维生素C的添加量达到500.0 mg/kg(Ⅴ组)以上时,七彩神仙鱼仔幼鱼血清替代补体活力、溶菌酶活力和白细胞吞噬活性均开始显著高于对照组(P<0.05),且同一水平随时间的增加而升高。添加量达1 500.0 mg/kg(Ⅷ组)之后,随着饲料中维生素C添加量的进一步增加,各组数据反而呈下降趋势。可见饲料中适量添加维生素C能够提高七彩神仙鱼仔幼鱼的非特异性免疫力,维生素C缺乏和过量都会对其带来不利影响。
关键词:  七彩神仙鱼  维生素C  非特异性免疫
DOI:
分类号:
基金项目:宁波市科技计划项目(2004C100057)资助
Influence of dietary vitamin C on non-specific immunity of larval and juvenile Symphysodon aequifasciatus
Abstract:
A feeding trial was conducted to evaluate effects of dietary vitamin C on non specific immunity of larval and juvenile Symphysodon aequifasciatus with supplement of vitamin C in feed(0-2000.0 mg/kg). When the supplementation ratio reached 500.0 mg/kg(group Ⅴ), the alternative complement pathway (ACP) activity, serum lysozyme activity, phagocytosis index(PI) and phagocytosis percentage(PP) of the fish were significantly higher than that of the control (P<0.05), and they increased over time in the same level. And then the non-specific immune index dropped with the increase of dietary vitamin C above 1500.0 mg/kg (group Ⅷ). It was found that proper level of vitamin C supplement in feed may enhance the nonspecific immunity of the larval and juvenile fish, while lack of vitamin C or over-dose may negatively affect the fish health.
Key words:  Symphysodon aequifasciatus  Vitamin C  Non-specific immunity