| 摘要: |
| 采用叶绿素荧光技术，以光系统Ⅱ最大荧光产量（Fv/Fm）为指标，结合形态显微观察，研究了GeO2在海带幼孢子体培育过程中菱形藻污染的抑制效应。结果表明，1）对于菱形藻，当GeO2浓度在5 mg/L以上时，对其Fv/Fm值有显著影响，达到或超过15 mg/L时菱形藻趋于死亡。2）对于海带幼孢子体，GeO2浓度在20 mg/L以上时，对其Fv/Fm值有显著影响，达到或超过30 mg/L时其趋于死亡。3）对于受到菱形藻污染的海带幼孢子体，GeO2浓度在15 mg/L以上时对其Fv/Fm值有显著影响，达到或超过20 mg/L时其趋于死亡。综合来看，当GeO2浓度为5～15 mg/L时，菱形藻光合作用受到较强的抑制，而对海带幼孢子体影响不大。 |
| 关键词: GeO2 海带幼孢子体 菱形藻 叶绿素荧光技术 |
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| 基金项目:国家“863”项目（2012AA10A406）和海带行业专项（B22201002）共同资助 |
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| Suppressive effect of germanium dioxide on diatom contamination during cultivation of Saccharina japonica young sporophytes |
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| Abstract: |
| In the present study, using the chlorophyll fluorescence technique, the suppressive effect of germanium dioxide (GeO2)on Nitzschia sp. contamination during the cultivation of Saccharina japonica seedling was investigated with the photosystem II maximum fluorescence yield (Fv/Fm) as the index. The results showed that: 1) When the concentration was above 5mg/L, GeO2 affected Fv/Fm value of Nitzschia sp. significantly, whereas when the concentration was 15mg/L or above，GeO2 had lethal effect on Nitzschia sp.; 2) To the young sporophytes of S. japonica, GeO2 at concentrations of 20mg/L or above affected the Fv/Fm value significantly, whereas GeO2 at concentrations of 30mg/L or above had lethal effect on young sporophytes; 3) To the young sporophytes contaminated by Nitzschia sp.，the Fv/Fm value was affected significantly by GeO2 at concentrations of 15 mg/L or above , while the contaminated young sporophytes died when GeO2 concentration was 20 mg/L or above. Comparatively, GeO2 at concentrations of 5～15 mg/L could suppress the photosynthesis of Nitzschia sp. significantly, but only had a minor effect on photosynthesis of S. japonica young sporophytes. Therefore, GeO2 at concentrations of 5～15 mg/L could prevent the Nitzschia sp. contamination during the cultivation of S. japonica young sporophytes. The results obtained from the present study could provide the basis for preventing the Nitzschia sp. contamination during the cultivation of S. japonica young sporophytes. |
| Key words: Germanium dioxide Saccharina japonica sporophytes Nitzschia sp. Chlorophyll fluorescence technique |
