| 摘要: |
| 以刺参龙须菜混合养殖生态系统为研究对象，研究了系统中细菌数量的变化规律，沉积物中细菌群落结构和多样性的变化。结果表明，实验期间，刺参单养组及不同配比的刺参龙须菜混养组，围隔内底层水体中细菌数量均呈增高趋势，细菌数量在2.14×106~4.27×106cells/ml之间。不同刺参龙须菜配比的养殖系统内沉积物中的细菌数量在1.55×108~3.39×108cells/gdw之间，比水体中的细菌数量高两个数量级。DGGE图谱的UPGMA树状结构聚类分析图表明，不同取样时间（6月17日、7月7日）的沉积物细菌组成差异较大。系统发育分析表明，刺参龙须菜混养围隔内沉积物的优势细菌主要归属于拟杆菌纲（Bacteroidetes）、γ-变形菌纲（γ-proteobacteria）、δ-变形菌纲（δ-proteobacteria）和α-变形菌纲（α-proteobacteria）。 |
| 关键词: 刺参 龙须菜 细菌 混养 |
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| 基金项目:国家自然科学基金青年基金（41106145）、国家科技支撑计划项目(2011BAD13B02)、国家863计划（2012AA10A412）、中国博士后科学基金(20090451355)和山东省博士后创新资金项目（200902007） |
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| The amount and community composition of bacteria in the co-culture system of Apostichopus japonicus and Gracilaria lemaneiformis |
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| Abstract: |
| The variation of amount and community composition of bacteria in the sea water and sediments of monoculture (Apostichopus japonicus) and co-culture systems (A. japonicus co-cultured with Gracilaria lemaneiformis) were evaluated. The total numbers of bacteria (2.14×106~4.27 ×106cells/ml) in the bottom water of the monoculture and co-culture systems increased during the period of the experiment. The bacteria numbers in the surface sediments ranged from 1.55 ×108 to 3.39 ×108cells/gdw in the systems, which were much higher than those in the bottom sea water. UPMGA cluster analysis of DGGE fingerprint from sediments revealed the bacterial community in the same sampling date had higher similarity than those in different sampling dates. Phylogenetic analysis showed that the dominant bacteria of the sediment in the monoculture and co-culture systems belong to α-proteobacteria, γ-proteobacteria, δ-proteobacteria and bacteroidetes. |
| Key words: Apostichopus japonicus Gracilaria lemaneiformis Bacteria Co-culture |
