| 摘要: |
| 采用口服给药、不同时间点采样的方法研究了氟苯尼考在牙鲆体内的残留消除规律，采用高效液相色谱法测定了氟苯尼考在牙鲆组织中的含量。结果表明：1）牙鲆连续5 d口服剂量为50 mg/kg的氟苯尼考后，药物在各组织中的分布情况为血液>内脏团>肌肉，随后各组织中氟苯尼考的浓度逐渐下降。6 d后各个组织药物浓度趋于平稳，而肝肾中的氟苯尼考浓度在10 d和14 d时有回升现象。2）T1/2为32.09～40.73 h，说明口服氟苯尼考在牙鲆体内消除较快，残留较少，但肾脏和肝脏组织中残留较为明显。3）探讨休药期的制定，如果只考虑可食用组织，且最大残留限量为0.1μg/g，在本试验的养殖环境下推算，牙鲆养殖过程中的平均温度为16～18℃时，休药期≥18 d。用温度与时间乘积表示则不低于324度•日。 |
| 关键词: 氟苯尼考 高效液相色谱 牙鲆 消除半衰期 消除规律 |
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| 基金项目:国家海洋局海洋环境评价专项[DOMEP(MEA)-02、DOMEP (MEA)-01-01]和中央级公益性科研院所基本科研业务费（20603022011002） |
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| Elimination of florfenicol residue in Paralichthys olivaceus (T&S) |
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| Abstract: |
| The elimination of florfenicol in Paralichthys olivaceus (T&S) was studied by oral administration. The concentrations of florfenicol in P. olivaceus (T&S) were determined by HPLC. The results showed that: 1）After oral administration at a dose of 50mg/kg for five consecutive days, the concentration of florfenicol in the tissues are ranked as: blood>visceral mass>muscle. The drug concentration in tissues stabilized after 6d, but florfenicol concentration in liver and kidney at 10d and 14d showed rebound phenomenon. 2）T1/2 were 32.09～40.73h , which showed the faster elimination and the less residual amount of florfenicol in the body. However, residual concentrations in the kidney and liver were more obvious. 3) To explore the development of off-drug period, considering edible parts and MRL standard(0.1 μg /g), under the culture conditions of P.olivaceus: average temperature of 16~18℃, off-drug period ≥18d, the product of temperature and time is no less than 324 degree•days. |
| Key words: Florfenico1 High Performance Liquid Chromatography Paralichthys olivaceus Half life of elimination Elimination regularity |
