| 摘要: |
| 在基础饲料中添加不同剂量(1%、3%、5%)的微胶囊剂型黄芪多糖(Astragalus polysaccharudes,APS) (载药量为17%)，制成3种配合饲料，连续投喂初始体重为22.0±1.0g的刺参Apostichopus japonicus 35d，探讨微胶囊剂型黄芪多糖（以下简称“微胶囊”）对刺参生长性能、非特异性免疫及抗病力的影响。结果显示，35d后刺参最高特定生长率(Specific growth rate, SGR)为0.51%/d(5%添加组)(P<0.05)；微胶囊添加量为3%时免疫效果最佳，刺参体腔液中酸性磷酸酶ACP、超氧化物歧化酶SOD、髓过氧化物酶MPO活性显著高于对照组(P<0.05)。试验期间每隔7d测定刺参体腔液样品，其中ACP活性在第28天最高，为11.2U/100ml (P<0.05)；SOD活性在第21天最高，为63.3U/ml(P<0.05)；MPO活性在第21天最高，为40 MPO单位/ml(P>0.05)；免疫试验结束后对刺参进行灿烂弧菌Vibrio splendidus攻毒实验，14d内观察得出，3%微胶囊添加组累积死亡率明显低于对照组 (P<0.05)。由此可得，微胶囊剂型黄芪多糖对仿刺参生长性能、免疫力及抗病力方面均具有显著的增强效果，以3%添加量效果最佳，且添加量、作用时间、免疫效果之间存在相关性。 |
| 关键词: 微胶囊 黄芪多糖刺参 生长 免疫 抗病力 |
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| 基金项目:山东省农业重大应用技术创新课题（鲁财农指\[2010\]58号）、海洋公益性行业科研专项经费项目200905020、山东省科技发展计划项目（2010GHY10501）和山东省优秀中青年科学家科研奖励基金项目(BS2010NY021) |
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| Impact of APS microcapsules on growth,immunity and disease resistance of sea cucumber Apostichopus japonicus |
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| Abstract: |
| The study was conducted to investigate the impact of Astragalus polysaccgarudes(APS) microcapsules (drug loading 17%) on the growth, nonspecific immune indices and resistance of Apostichopus japonicas against Vibrio splendidus infection.APS microcapsules were added at graded levels (1%, 3%, and 5%) to the basal diet to formulate three practical compound diets. Continuous 35-day feeding trials were carried out on A. japonicus at initial body weight of 22.0±1.0g. After feeding the diets for 35 days, specific growth rate (SGR) for the 5% treatment was 0.51%/d, which was significantly higher than the control. The activities of serum acid phosphatase (ACP), superoxide dismutase (SOD), and myeloperoxidase (MPO) increased significantly (P<0.05) in the 3% treatment. During the experiment these factors were determined at 7-day intervals. The activity of ACP was 11.2U/100ml at 28d during the experiment (P<0.05), the activity of SOD was 63.3U/ml at 21d (P<0.05), and the activity of MPO was 40MPO unit/ml at 21d (P>0.05). After the feeding trial, a V. splendidus injection challenge was given to the sea cucumbers to test the effects of APS microcapsule. The 3% treatment showed significantly better survival than the control (P<0.05). It is suggested that supplementation of 3% APS microcapsules has the greatest effects on growth, immunity and disease resistance in A. japonicas against V.splendidus. In addition, there was a direct relationship between the level and time of treatment, and the immunity. |
| Key words: Microcapsule Astragalus polysaccharide Apostichopus japonicas Growth Immune Disease resistance |
